標(biāo)題NQO1 Catalog Number：OM628927

提供者：上海篤瑪生物科技有限公司發(fā)布時(shí)間：2024/9/24 閱讀次數(shù)：45次 >>進(jìn)入該公司展臺(tái)

Product Name	NQO1
Antibody Type	Primary Antibodies
Product description	NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals ａnd radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression ａnd coordinated induction of NQO1. ARE activation by tert-butylhydroqui is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B ａnd Jun-D bind to the ARE ａnd regulate expression ａnd induction of NQO1 gene. Maf-Maf homodimers ａnd possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE ａnd do not repress NQO1 transcription. More
Immunogen	Recombinant protein within human NQO1 1-250 aa.

Alternative Names	Azoreductase antibody Cytochrome b 5 reductase antibody DHQU antibody DIA 4 antibody DIA4 antibody Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody Diaphorase (NADH/NADPH) antibody Diaphorase 4 antibody Dioxin inducible 1 antibody DT diaphorase antibody DT-diaphorase antibody DTD antibody Menadione reductase antibody NAD(P)H dehydrogenase [qui] 1 antibody NAD(P)H dehydrogenase qui 1 antibody NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody NAD(P)H qui dehydrogenase 1 antibody NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody NAD(P)H:menadione oxidoreductase 1 antibody NAD(P)H:Qui acceptor oxidoreductase type 1 antibody NAD(P)H:qui oxidoreductase 1 antibody NAD(P)H:qui oxireductase antibody NMOR 1 antibody NMOR I antibody NMOR1 antibody NMORI antibody NQO 1 antibody NQO1 antibody NQO1_HUMAN antibody Phylloqui reductase antibody QR 1 antibody QR1 antibody Qui reductase 1 antibody More
Molecular Weight(MW)	31 kDa
Cellular Localization	Cylasm.

SwissProt ID	P15559 Q64669 P05982

Application

Fig1: Western blot analysis of NQO1 on different cell lysates using anti-NQO1 antibody at 1/1,000 dilution. Positive control： Lane1: Siha Lane2: A549
Application

Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-NQO1 antibody. Counter stained with hematoxylin.
Application

Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-NQO1 antibody. Counter stained with hematoxylin.
Application

Fig4: Flow cytometric analysis of Siha cells with NQO1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Positive Control	Siha, A549, rat kidney tissue, mouse colon tissue.
Application Notes	WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:50-1:100

Form	Liquid
Storage Instructions	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer	1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide

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