NAD(P)H:qui oxidoreductase 1 (NQO1) ａnd NRH:qui oxidoreductase (NQO2) are flavoproteins that catalyze the metabolic detoxification of quis ａnd their derivatives to hydroquis, using either NADH or NADPH as the electron donor. This protects cells against qui-induced oxidative stress, cytotoxicity, ａnd mutagenicity. Many tumors overexpress NQO1, which is an obligate two-electron reductase that deactivates toxins ａnd activates bioreductive anticancer drugs. NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals ａnd radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression ａnd coordinated induction of NQO1. ARE activation by tert-butylhydroqui is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B ａnd Jun-D bind to the ARE ａnd regulate expression ａnd induction of NQO1 gene. Maf-Maf homodimers ａnd possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE ａnd do not repress NQO1 transcription.
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recombinant protein

WBICC/IFIPFC

HumanMouseRat