標(biāo)題ITK [A6-G12] Catalog Number：OM627718

提供者：上海篤瑪生物科技有限公司發(fā)布時(shí)間：2024/9/29 閱讀次數(shù)：25次 >>進(jìn)入該公司展臺(tái)

Product Name	ITK [A6-G12]
Antibody Type	Primary Antibodies
Product description	The Tec family of non-receptor tyrosine kinases is composed of six proteins designated Tec, Emt (also known as Itk or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) ａnd Dsrc28C. Four alternatively spliced forms of Tec are found to be expressed broadly in cells Emt gene product associates with CD28 ａnd becomes activated subsequent to CD28 ligation. Btk is necessary for proper B cell development, ａnd mutations in the gene encoding Btk have been associated with families suffering from X-linked agammaglobulinemia, also referred to as Bruton＇s disease. The Bmx protein shares a high degree of homology with Btk ａnd seems to be expressed at highest levels in the heart. Txk expression is T cell-specific, while expression of the Drosophila Tec homolog, Dsrc28C, is developmentally regulated. More
Immunogen	Recombinant protein

Alternative Names	EMT antibody Homolog of mouse T cell itk/tsk antibody IL 2 inducible T cell kinase antibody IL2 inducible T cell kinase antibody Interleukin 2 inducible T cell kinase antibody Itk antibody ITK_HUMAN antibody Kinase EMT antibody LPFS1 antibody LYK antibody MGC126257 antibody MGC126258 antibody PSCTK 2 antibody PSCTK2 antibody T cell specific kinase antibody T-cell-specific kinase antibody TSK antibody Tyrosine protein kinase antibody Tyrosine protein kinase ITK/TSK antibody Tyrosine protein kinase Lyk antibody Tyrosine-protein kinase ITK/TSK antibody Tyrosine-protein kinase Lyk antibody More
Molecular Weight(MW)	72 kDa
Cellular Localization	Cylasm.

SwissProt ID	Q08881

Application

Fig1: Western blot analysis of ITK on human ITK recombinant protein using anti-ITK antibody at 1/1,000 dilution.
Application

Fig2: Western blot analysis of ITK on Jurkat cell lysate using anti-ITK antibody at 1/1,000 dilution.
Application

Fig3: ICC staining ITK (green) in Jurkat cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application

Fig4: Flow cytometric analysis of Jurkat cells with ITK antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).

Positive Control	Human ITK recombinant protein, Jurkat.
Application Notes	WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:100-1:200

Form	Liquid
Storage Instructions	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer	1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

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