您的位置:首頁 > 技術(shù)文獻(xiàn) > 產(chǎn)品說明 > PHB Catalog Number:OM640928
| Product Name | PHB |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human PHB expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 More IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRatMonkey |
| Alternative Names | |
|---|---|
| Molecular Weight(MW) | 30kDa |
| Entrez Gene | 5245 |
|---|

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Figure 1: Western blot analysis using PHB mAb against human PHB (AA: 68-259) recombinant protein. (Expected MW is 46.7 kDa)

Figure 2: Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.

Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PHB mouse mAb with DAB staining.

Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using PHB mouse mAb with DAB staining.

Figure 5: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PHB mouse mAb with DAB staining.

Figure 6: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PHB mouse mAb with DAB staining.

Figure 7: Immunofluorescence analysis of NIH/3T3 cells using PHB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Figure 8: Flow cytometric analysis of MCF-7 cells using PHB mouse mAb (blue) and negative control (red).
| Application Notes | WB:1:250~1:1000 More IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
關(guān)鍵詞:穩(wěn)定性強(qiáng) 特異性強(qiáng) 靈敏度高
① 凡本網(wǎng)注明"來源:易推廣"的所有作品,版權(quán)均屬于易推廣,未經(jīng)本網(wǎng)授權(quán)不得轉(zhuǎn)載、摘編或利用其它方式使用。已獲本網(wǎng)授權(quán)的作品,應(yīng)在授權(quán)范圍內(nèi)
使用,并注明"來源:易推廣"。違者本網(wǎng)將追究相關(guān)法律責(zé)任。② 本信息由注冊(cè)會(huì)員:上海篤瑪生物科技有限公司 發(fā)布并且負(fù)責(zé)版權(quán)等法律責(zé)任。

易推廣客服微信
