標(biāo)題PHB Antibody Catalog Number：OM287957

提供者：上海篤瑪生物科技有限公司發(fā)布時(shí)間：2024/9/26 閱讀次數(shù)：25次 >>進(jìn)入該公司展臺(tái)

Product Name	PHB Antibody
Antibody Type	Primary Antibodies
Product description	Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation ａnd may be a tumor suppressor. Mutations have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3＇ untranslated region.1) Gregory-Bass,R.C., Int. J. Cancer 122 (9), 1923-1930 (2008) More
Immunogen	This PHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PHB.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
Tested Applications	FACSIFIHC-PWB
	For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50:
Species Reactivity	Human
Concentration	1 mg/ml

Gene Symbol	PHB
Alternative Names	Prohibitin PHB
Molecular Weight(MW)	30 kDa
Sequence Similarities	Predicted species reactivity based on immunogen sequence: Bovine

Entrez Gene	5245
Protein Accession	P35232

Application

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application

Confocal immunofluorescent analysis of PHB Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Application

Western blot analysis of PHB Antibody in Hela cell line lysates (35ug/lane)
Application

Western blot analysis of PHB using rabbit polyclonal PHB Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHB gene.
Application

Formalin-fixed ａnd paraffin-embedded human hepatocarcinoma tissue reacted with PHB antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Application Notes	For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50:

Form	Liquid
Storage Instructions	Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

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