標(biāo)題Gab1 Antibody Catalog Number：OM233623

提供者：上海篤瑪生物科技有限公司發(fā)布時(shí)間：2024/9/14 閱讀次數(shù)：51次 >>進(jìn)入該公司展臺(tái)

Product Name	Gab1 Antibody
Antibody Type	Primary Antibodies

Alternative Names	GAB1 GRB2-associated-binding protein 1 GRB2-associated binder 1 Growth factor receptor bound protein 2-associated protein 1 More
Molecular Weight(MW)	76616 Da
Function	Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) ａnd the insulin receptor (INSR)
Tissue Specificity	This Gab1 antibody is generated from mice immunized with a GST fusion protein encoding full length human GAB1.

Entrez Gene	2549

Application

Wild-type (+/+) ａnd Gab1-/- (-/-) cells were heat shocked (HS) at 42擄C for 1 h or irradiated with UV-B light (400 J/m2) ａnd then incubated at 37? for 1 h. Cell lysates were prepared ａnd subjected to immunoprecipitation (IP) with an anti-JNK2 antibody. The immunoprecipitates were resolved by SDS- PAGE ａnd immunoblotted (IB) with anti-Gab1 antibody. Data courtesy of Dr. GS Feng at The Burnham Institute.
Application

Rescue of the JNK pathway by expression of wild-type Gab1 in Gab1-/- cells. Gab1-/- cells were transiently transfected with 1, 2, or 4 of the expression construct for human Gab1 cDNA, using the GenePORTER 2 transfection reagent (Gene Therapy Systems Inc.). After incubation, the cells were irradiated with UV-B light at 400 J/m2 or left untreated. Exogenous expression of Gab1 in transfected cells was confirmed by anti-Gab1 immunoblot analysis with wild-type cells as positive control. +/+, wild-type cells; -/-, Gab1-/- cells. (Mol. Cell. Biol. 2004 Feb 15;24(4):1531-1539)

Application Notes	WB~~1:1000:

Form	Liquid
Storage Instructions	For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high ａnd low pH buffers ａnd neutralized immediately, followed by dialysis against PBS.

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