WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, ａnd a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, ａnd is a member of the NLRP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, ａnd it plays a role in the regulation of inflammation, the immune response, ａnd apoptosis. The SARS-CoV 3a protein, a transmembrane pore-forming viroporin, has been shown to activate the NLRP3 inflammasome via the formation of ion channels in macrophages. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous ａnd articular (CINCA) syndrome, neonatal-onset multisystem inflammatory disease (NOMID), keratoendotheliitis fugax hereditarian, ａnd deafness, autosomal dominant 34, with or without inflammation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5＇ UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5＇ UTR splice patterns are biologically valid. [provided by RefSeq, Aug 2020]

As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity ａnd inflammation. In response to pathogens ａnd other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD ａnd CASP1 (and possibly CASP4 ａnd CASP5). Recruitment of proCASP1 to the inflammasome promotes its activation ａnd CASP1-catalyzed IL1B ａnd IL18 maturation ａnd secretion in the extracellular milieu

WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500