schreiber教授因發(fā)明pam系列調(diào)制葉綠素?zé)晒鈨x而獲得首屆國際光合作用協(xié)會（ispr）創(chuàng)新獎
利用調(diào)制葉綠素?zé)晒饧夹g(shù)，測量野外自然水體或培養(yǎng)的微藻樣品的光合作用（葉綠素?zé)晒庹T導(dǎo)加淬滅分析、光響應(yīng)曲線等），也可測量葉綠素含量，是進(jìn)行野外光合作用研究的良好工具。除了測浮游植物外，可擴(kuò)展探頭測量附著藻類或大型藻類。除了取水樣到樣品杯中測量外，可擴(kuò)展探頭進(jìn)行水下原位、連續(xù)測量，特別適合于連續(xù)監(jiān)測海洋、湖泊、水庫、河流等水體的葉綠素含量以及光合活性。
功能：
1）可單機(jī)操作，可連接電腦操作
2）可測葉綠素?zé)晒庹T導(dǎo)曲線并進(jìn)行淬滅分析
3）可測光響應(yīng)曲線和快速光曲線（rlc）
4）可測量水樣的量子產(chǎn)量（光合效率）和相對電子傳遞速率（光合速率）
5）51個內(nèi)置模式菜單，方便參數(shù)設(shè)置和標(biāo)準(zhǔn)測量
6）靈敏度高，檢測限為0.1 μg l-1 chl
7）三種配置：
系統(tǒng)i用于浮游植物研究；系統(tǒng)ii用于附著藻類和大型藻類研究；系統(tǒng)iii用于連續(xù)監(jiān)測水體光合作用
8）主機(jī)為通用控制單元pam-control，可擴(kuò)展為microscopy-pam和microfiber-pam
|
通用控制單元pam-control |
測量參數(shù)：
fo、fm、fv/fm、f、fm"、δf/fm"、fo"、qp、qn、npq、retr和par等。
應(yīng)用領(lǐng)域：
水生生物學(xué)、水域生態(tài)學(xué)、海洋學(xué)、湖沼學(xué)、微藻生理學(xué)、微藻抗逆性、水質(zhì)管理、飲用水源監(jiān)測、極地藻類研究、環(huán)境科學(xué)、生態(tài)毒理學(xué)等。
| 系統(tǒng)組成 | |
系統(tǒng)i
浮游植物版 | 系統(tǒng)ii
附著藻類/大型藻類版 | 系統(tǒng)iii
連續(xù)監(jiān)測版 |
| 
| 
|
| 野外現(xiàn)場自然水體的光合作用檢測、葉綠素含量測定；室內(nèi)培養(yǎng)的微藻樣品的生理特性研究等。 | 野外現(xiàn)場附著藻類（如底泥中的藻類）、大型海藻的光合活性測量；室內(nèi)大型海藻生理特性研究。 | 野外現(xiàn)場水體光合活性、葉綠素含量的連續(xù)測定，包括垂直梯度分布和橫向分布。 |
| 可選附件1：攪拌器，可置于系統(tǒng)i的上部對水樣進(jìn)行攪拌，帶內(nèi)置電池 
| | |
可選附件2：球狀微型光量子探頭，可放入系統(tǒng)i的樣品杯中測量par，也可放入水面下測量水體光場
| | |
技術(shù)參數(shù)：
測量光：紅色led，650 nm，最大光強(qiáng) <1 μmol m-2 s-1
光化光：紅色led，660 nm，最大光強(qiáng)2000 μmol m-2 s-1 par
飽和脈沖：紅色led，660 nm，光強(qiáng)4000 μmol m-2 s-1 par
遠(yuǎn)紅光：led，730 nm
信號檢測：光電倍增管，檢測波長 > 710 nm，選擇性鎖相放大器（專利設(shè)計），過載保護(hù)功能
部分文獻(xiàn)
[1] drath m, kloft n, batschauer a, marin k, novak j, forchhammer k. ammonia triggers photodamage of photosystem ii in the cyanobacterium synechocystis sp. strain pcc 6803. plant physiology 2008:in press.
[2] gao k, li p, watanabe t, helbling ew. combined effects of ultraviolet radiation and temperature on morphology, photosynthesis, and dna of arthrospira (spirulina) platensis (cyanophyta). journal of phycology 2008:in press.
[3] hill r, ralph pj. impact of bleaching stress on the function of the oxygen evolving complex of zooxanthellae from scleractinian corals. journal of phycology 2008;44 (2):299-310.
[4] mojaat m, foucault a, pruvost j, legrand j. optimal se-lection of organic solvents for biocompatible extraction of β-carotene from dunaliella salina journal of biotechnology 2008;133 (4):433-41.
[5] bailey s, melis a, mackey krm, cardol p, finazzi g, dijken gv, berge gm, arrigo k, shrager j, grossman a. alternative photosynthetic electron flow to oxygen in marine synechococcus biochimica et biophysica acta 2007;1777 (3):269-76.
[6] ekelund nga, aronsson ka. changes in chlorophyll a fluorescence in euglena gracilis and chlamydomonas reinhardtii after exposure to wood-ash environmental and experimental botany 2007;59 (1):92-8.
[7] geoffroy l, gilbin r, simon o, floriani m, adam c, pradines c, cournac l, garnier-laplace j. effect of selenate on growth and photosynthesis of chlamydomonas reinhardtii aquatic toxicology 2007;83 (2):149-58.
[8] hill r, ralph pj. post-bleaching viability of expelled zooxanthellae from the scleractinian coral pocillopora damicornis. marine ecology progress series 2007;352:137-44.
[9] kromkamp jc, perkins r, dijkman n, consalvey m, andres m, reid rp. resistance to burial of cyanobacteria in stromatolites. aquatic microbial ecology 2007;48 (2):123-30.
[10] kudela rm, ryan jp, blakely md, lane jq, peterson td. linking the physiology and ecology of cochlodinium to better understand harmful algal bloom events: a comparative approach harmful algae 2007;7 (3):278-92.
[11] leu e, falk-petersen s, hessen do. ultraviolet radiation negatively affects growth but not food quality of arctic diatoms. limnology and oceanography 2007;52 (2):787-97.
[12] mcminn a, ryan kg, ralph pj, pankowski a. spring sea ice photosynthesis, primary productivity and biomass distribution in eastern antarctica, 2002-2004 marine biology 2007;151 (3):985-95.
[13] perkins rg, kromkam jc, reid rp. importance of light and oxygen for photochemical reactivation in photosynthetic stromatolite communities after natural sand burial. marine ecology progress series 2007;349:23-32.
[14] ralph pj, ryan kg, martin a, fenton g. melting out of sea ice causes greater photosynthetic stress in algae than freezing in. journal of phycology 2007;43 (5):948-56.
[15] richter p, helbling w, streb c, häder d-p. par and uv effects on vertical migration and photosynthesis in euglena gracilis. photochemistry and photobiology 2007;83 (4):818-23.
[16] roleda my, zacher k, wulff a, hanelt d, wiencke c. photosynthetic performance, dna damage and repair in gametes of the endemic antarctic brown alga ascoseira mirabilis exposed to ultraviolet radiation. austral ecology 2007;32 (8):917-26.
[17] schmitt-jansen m, altenburger r. the use of pulse-amplitude modulated (pam) fluorescence-based methods to evaluate effects of herbicides in microalgal systems of different complexity toxicological and environmental chemistry 2007;89 (4):665-81.
[18] veldhuis mjw, timmermans kr. phytoplankton dynamics during an in situ iron enrichment experiment (eisenex) in the southern ocean: a comparative study of field and bottle incubation measurements. aquatic microbial ecology 2007;47 (2):191-208.
[19] wulff a, zacher k, hanelt d, al-handal a, wiencke c. uv radiation - a threat to antarctic benthic marine diatoms? antarctic science 2007;20:13-20.
[20] zacher k, roleda my, hanelt d, wiencke c. uv effects on photosynthesis and dna in propagules of three antarctic seaweeds (adenocystis utricularis, monostroma hariotii and porphyra endiviifolium) planta 2007;225 (6):1505-16.
[21] 梁英, 尹翠玲, 江新琴, 于云芝. 硅濃度對纖細(xì)角毛藻和三角褐指藻生長及葉綠素?zé)晒馓匦缘挠绊? 海洋水產(chǎn)研究 2007;28 (5):89-94.
[22] 尹翠玲, 梁英, 張秋豐. 磷濃度對球等鞭金藻3011和8701葉綠素?zé)晒馓匦约吧L的影響. 海洋湖沼通報 2007:88-95.
[23] cosgrove j, borowitzka m. applying pulse amplitude modulation (pam) fluorometry to microalgae suspensions: stirring potentially impacts fluorescence. photosynthesis research 2006;88 (3):343-50.
[24] leu e, færøvig pj, hessen do. uv effects on stoichiometry and pufas of selenastrum capricornutum and their consequences for the grazer daphnia magna. freshwater biology 2006;51 (12):2296-308.
[25] peers g, price nm. copper-containing plastocyanin used for electron transport by an oceanic diatom nature 2006;441:341-4.
[26] roleda my, hanelt d, wiencke c. exposure to ultraviolet radiation delays photosynthetic recovery in arctic kelp zoospores. photosynthesis research 2006;88 (3):311-22.
[27] roleda my, wiencke c, lüder2 uh. impact of ultraviolet radiation on cell structure, uv-absorbing compounds, photosynthesis, dna damage, and germination in zoospores of arctic saccorhiza dermatodea journal of experimental botany 2006;57 (14):3847-56.
[28] 梁英, 馮力霞, 尹翠玲, 曹春暉. 高溫脅迫對三角褐指藻和纖細(xì)角毛藻葉綠素?zé)晒鈩恿W(xué)的影響. 中國海洋大學(xué)學(xué)報 2006;36 (3):427-33.
[29] 梁英, 馮力霞, 田傳遠(yuǎn), 尹翠玲. 鹽脅迫對塔胞藻生長及葉綠素?zé)晒鈩恿W(xué)的影響. 中國海洋大學(xué)學(xué)報 2006;36 (5):726-32.
[30] 冉春秋, 張衛(wèi), 虞星炬, 金美芳, 鄧麥村. 解偶聯(lián)劑cccp對萊茵衣藻光照產(chǎn)氫過程的調(diào)控. 高等學(xué)�；瘜W(xué)學(xué)報 2006;27 (1):62－6.
[31] barranguet c, veuger b, van beusekom sam, marvan p, sinke jj, admiraal w. divergent composition of algal-bacterial biofilms developing under various external factors. european journal of phycology 2005;40 (1):1-8.
[32] mcminn a, pankowski a, delfatti t. effect of hyperoxia on the growth and photosynthesis of polar sea ice microalgae. journal of phycology 2005;41 (4):732-41.
[33] mcminn a, hirawake t, hamaoka t, hattori h, fukuchi m. contribution of benthic microalgae to ice covered coastal ecosystems in northern hokkaido, japan. journal of the marine biological association of the uk 2005;85:283-9.
[34] mcminn a, sellah s, llah wawa, mohammad m, merican fms, omar wmw, samad f, cheah w, idris i, sim yk, wong ws, tan sh, yasin z. quantum yield of the marine benthic microflora of near-shore coastal penang, malaysia. marine and freshwater research 2005;56 (7):1047-53.
[35] ralph pj, mcminn a, ryan kg, ashworth c. short-term effect of temperature on the photokinetics of microalgae from the surface layers of antarctic pack ice. journal of phycology 2005;41 (4):763-9.
[36] serôdio j, vieira s, cruz s, barroso f. short-term variability in the photosynthetic activity of microphytobenthos as detected by measuring rapid light curves using variable fluorescence. marine biology 2005;146 (5):903-14.
[37] mcminn a, hegseth en. quantum yield and photosynthetic parameters of marine microalgae from the southern arctic ocean, svalbard. journal of the marine biological association of the united kingdom 2004;84:865-71.
[38] ryan kg, ralph p, mcminn a. acclimation of antarctic bottom-ice algal communities to lowered salinities during melting. polar biology 2004;27:679-86.
[39] jones rj, heyward aj. the effects of produced formation water (pfw) on coral and isolated symbiotic dinoflagellates of coral. marine and freshwater research 2003;54 (2):153-62.
[40] sauer jr, schreiber u, schmid r, völker u, forchhammer k. nitrogen starvation-induced chlorosis in synechococcus pcc 7942. low-level photosynthesis as a mechanism of long-term survival. plant physiology 2001;126:233-43.
