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齊一生物現(xiàn)貨供應(yīng)人ELISA試劑盒，大鼠ELISA試劑盒，小鼠ELISA試劑盒等現(xiàn)貨充足，購(gòu)買任意ELISA試劑盒都享受免費(fèi)代測(cè)服務(wù)，更多進(jìn)口原裝品牌試劑盒供應(yīng)齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人巨噬細(xì)胞集落刺激因子(M-CSF)ELISA試劑盒現(xiàn)貨】酶法檢測(cè)試驗(yàn)所需自備物品:

1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

【人巨噬細(xì)胞集落刺激因子(M-CSF)ELISA試劑盒現(xiàn)貨】操作注意事項(xiàng)：

1.試劑盒應(yīng)按標(biāo)簽說(shuō)明書儲(chǔ)存，使用前恢復(fù)到室溫30分鐘后方可使用。稀稀過(guò)后的標(biāo)準(zhǔn)品應(yīng)丟棄，不可保存。

2.實(shí)驗(yàn)中不用的板條應(yīng)立即放回包裝袋中，密封保存，以免變質(zhì)。

3.不用的其它試劑應(yīng)包裝好或蓋好。不同批號(hào)的試劑不要混用.請(qǐng)?jiān)谠噭┖斜Ｙ|(zhì)前使用。

4.使用一次性的吸頭以免交叉污染，吸取終止液和底物A、B液時(shí)，避免使用帶金屬部分的加樣器。

5.使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。

6.洗滌酶標(biāo)板時(shí)應(yīng)充分拍干，不要將吸水紙直接放入酶標(biāo)反應(yīng)孔中吸水。

7.底物A應(yīng)揮發(fā)，避免長(zhǎng)時(shí)間打開蓋子。底物B對(duì)光敏感，避免長(zhǎng)時(shí)間暴露于光下。避免用手接觸，有毒。實(shí)驗(yàn)完成后應(yīng)立即讀取OD值。

8.加入試劑的順序應(yīng)一致，以保證所有反應(yīng)板孔溫育的時(shí)間一樣。

9.按照說(shuō)明書中標(biāo)明的時(shí)間、加液的量及順序進(jìn)行溫育操作。

【人巨噬細(xì)胞集落刺激因子(M-CSF)ELISA試劑盒現(xiàn)貨】齊一生物客戶寄標(biāo)本時(shí)需注明以下情況：

1、標(biāo)本編號(hào)；2、所測(cè)項(xiàng)目；3、是否做復(fù)孔；3、聯(lián)系方式；4、實(shí)驗(yàn)后標(biāo)本是否寄回。

客戶必知：

客戶應(yīng)對(duì)所提供的材料及信息負(fù)責(zé)，如因客戶提供的材料及信息不準(zhǔn)確而引起的實(shí)驗(yàn)延誤或經(jīng)濟(jì)損失由客戶承擔(dān)。

齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過(guò)程中必須堅(jiān)持全面質(zhì)量控制和全過(guò)程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒(méi)有PBS的話也可以用一下生理鹽水，還是用PBS

樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過(guò)濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人巨噬細(xì)胞集落刺激因子(M-CSF)ELISA試劑盒現(xiàn)貨】齊一生物供應(yīng)，以上資料供參考如有疑問(wèn)需要詳細(xì)資料請(qǐng)聯(lián)系我們客服人員，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

QY-x2152 CHO-K1中國(guó)倉(cāng)鼠卵巢細(xì)胞                                                

QY-x2153 ES-2人卵巢透明細(xì)胞癌細(xì)胞系                                                 

QY-x2154 H460人大細(xì)胞肺癌細(xì)胞系                                               

QY-x2155 HeLa 人宮頸癌細(xì)胞系                                                        

QY-x2156 HeLa 229人宮頸癌細(xì)胞系                                                 

QY-x2157 HL-60人髓性白血病細(xì)胞系（早幼粒白血�。�                                                        

QY-x2158 Jurkat 人白血病T細(xì)胞系                                                  

QY-x2159 K-562人類紅白血病細(xì)胞系                                                        

QY-x2160 MC3T3-E1小鼠成骨細(xì)胞                                                     

QY-x2161 A498人腎癌細(xì)胞                                                

QY-x2162 McCoy小鼠成纖維細(xì)胞                                                       【人巨噬細(xì)胞集落刺激因子(M-CSF)ELISA試劑盒現(xiàn)貨】

QY-x2163 MCF-7人乳腺癌細(xì)胞系                                                       

QY-x2164 MDA-MB-231人乳腺癌細(xì)胞系                                                  

QY-x2165 MDCK 犬上皮細(xì)胞                                                      

QY-x2166 NIH-3T3小鼠成纖維細(xì)胞                                                   

QY-x2167 OVCAR-3人卵巢癌細(xì)胞系                                                  

QY-x2168 Raji人B淋巴細(xì)胞瘤                                                  

QY-x2169 SKOV3卵巢癌細(xì)胞系                                                  

QY-x2170 Vero 非洲綠猴腎細(xì)胞                                               

QY-x2171 Vero E6非洲綠猴腎細(xì)胞                                                    

QY-x2172 BJ 人皮膚成纖維細(xì)胞                                                 

QY-x2173 MGC-803 人胃癌細(xì)胞系                                                    

QY-x2174 SGC-7901 人胃癌細(xì)胞系                                                    

QY-x2175 THC-8307 人高分化結(jié)腸腺癌細(xì)胞系                                                        

QY-x2176 MIAPaca-2 胰腺癌細(xì)胞系                                                 

QY-x2177 293A 人胚腎細(xì)胞系                                                    

QY-x2178 3AO 人卵巢癌細(xì)胞系                                                 【人巨噬細(xì)胞集落刺激因子(M-CSF)ELISA試劑盒現(xiàn)貨】

QY-x2179 M14 人肺癌細(xì)胞                                                 

QY-x2180 M21黑色素瘤細(xì)胞                                                       

QY-x2181 OMDA231人乳腺癌細(xì)胞                                                     

QY-x2182 HTB133人乳腺癌細(xì)胞                                                

QY-x2183 Caski 宮頸癌細(xì)胞                                                

QY-x2184 BJAB 人B細(xì)胞淋巴瘤細(xì)胞株                                                   

9013236  Tubing set, vacuum, 200, 0               CNY                             

9013224  Gripper set, V-handler, BR3000                 CNY                             

9013197  Tubing, L1 outlet, auto vac,BR8000 v1.00                CNY                             

9011702  Cable, PCON98 to AC-box (level sensor),          CNY                             

9011681  Dispenser drive, BR8000          CNY                             

132635     NeXtal Stock Zinc chloride (50)        0,1M Zinc chloride - 50mL (4 x 12,5 ml tube)  CNY                             

132578     NeXtal Stock PEG 2,000 DME (50)   50% w/v Polyethylene glycol 2,000 dimethyl ether - 50mL (4 x 12,5 ml tube)         CNY                             

800341     Cosmid End-Sequencing, w/ prep              CNY                             

800308     siRNA Hairpin Run w/ prep                CNY                             

800121     Verification Sequencing, w/ prep     "Double-stranded sequencing of a given sequence

QIAGEN® DNA template preparation

Design ａnd synthesis of walking primers

Data assembly ａnd sequence alignment

Accuracy >99.99%" CNY                             

132528     NeXtal Stock CAPSO untit. (50)         0.5M CAPSO - Untitrated - 50mL (4 x 12,5 ml tube)         CNY                             

132510     NeXtal Stock ADA untit. (50)   0.5M ADA - Untitrated - 50mL (4 x 12,5 ml tube)   CNY                             

764114     PAXgene Bone Marrow RNA Tubes (50)           CNY                              【人巨噬細(xì)胞集落刺激因子(M-CSF)ELISA試劑盒現(xiàn)貨】

679913     ipsogen RT Kit (33)            CNY                             

203643     HotStarTaq Plus Master Mix Kit (250)     "For 250 x 20 µl reactions: 3 x 0.85 ml HotStarTaq Plus Master Mix, containing 250 units of HotStarTaq Plus DNA Polymerase total, 1 x 0.55 ml CoralLoad Concentrate, 2 x 1.9 ml RNase-free Water

HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, ａnd performance as HotStarTaq Plus DNA Polymerase NOW in a ｍaster mix format, which offers even faster results ａnd added convenience."  CNY                             

201225     Taq PCR Core Kit (1000)   "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

201203     Taq DNA Polymerase (250)      "250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

180922     GeneRead Adapter L Set 1plex (12)                   CNY                             

180912     GeneRead Adapter I Set 1plex (12)          CNY                             

159214     QIAcard FTA Indicator Four Spots (25)              CNY                             

159201     QIAcard FTA One Spot (100)              CNY                             

9235523  Needle cleaning assembly P/N 4567-01            CNY                             

9235300  Plunger tip, syringe 2,5ml                  CNY                             

9235117  Fuse F4A, v1.00                  CNY                             

9022818  EZ1 Advanced XL Flip Cap Tube Rack                 CNY                             

9234889  Vacuum manifold, 96 seal                  CNY                             

9234862  System liquid container (2l Nalgene)                 CNY                             

9239555  PCBA, Laser Driver, Unlimited          CNY                             

9238664  Hinge                 CNY                              【人巨噬細(xì)胞集落刺激因子(M-CSF)ELISA試劑盒現(xiàn)貨】

9238662  Lid with window                CNY                             

9238659  Cooling compressor T2168GK, 60Hz                  CNY