1. 酶標儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【人B細胞活化因子受體(BAFF-R)ELISA試劑盒廠家】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

ELISA應(yīng)用：

ELISA檢測試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯(lián)免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認可及推崇,在歐美及中國獲得很大的推廣,尤其是國內(nèi)生化領(lǐng)域的長足發(fā)展.Elisa生物試驗是一種敏感性高,特異性強,重復(fù)性好的實驗診斷方法.由于其試劑穩(wěn)定、易保存,操作簡便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗的各領(lǐng)域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【人B細胞活化因子受體(BAFF-R)ELISA試劑盒廠家】齊一生物科技歡迎您訂購咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【人B細胞活化因子受體(BAFF-R)ELISA試劑盒廠家】齊一生物供應(yīng)的ELISA試劑盒ELISA實驗原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實驗（ELISA）.已知濃度的標準品、未知濃度的樣品加入微孔酶標板內(nèi)進行檢測.先將生物素標記的抗體同時溫育.洗滌后,加入親和素標記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標記物如酶標結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).

HBsAg試劑特點（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點的鼠復(fù)合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應(yīng)用ParlEhrich學(xué)說（PEI）HBsAg標準品,對ad標準品的靈敏度為0.05ng/ml對ay標準品靈敏度為0.025ng/ml

ELISA檢測試劑盒應(yīng)用定性夾心免疫檢測技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標準品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時會發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標準,O.D.值大于或等于Cut-Off值的樣品被認為是初試陽性. 【人B細胞活化因子受體(BAFF-R)ELISA試劑盒廠家】

以上資料僅供參考了解，不供于論文寫作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長期經(jīng)營ELISA試劑盒（免費代測）及抗體、分子學(xué)生物試劑盒、金標法檢測試劑盒、比色法檢測試劑盒、細胞菌株、培養(yǎng)基、細胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

QY-x1100 人胚肺成纖維細胞　HELF                                                  

QY-x1101 人低轉(zhuǎn)移肺癌細胞95-C                                                       

QY-x1102 人高轉(zhuǎn)移肺癌細胞95-D                                                      

QY-x1103 人肺腺癌細胞A2                                                 

QY-x1104 人支氣管上皮細胞HBE                                                       

QY-x1105 人支氣管上皮細胞16HBE                                                   

QY-x1106 人腎癌細胞Ketr-3                                               

QY-x1107 人腎癌細胞OS-RC-2                                                     

QY-x1108 人腎透明細胞腺癌細胞786-O                                                    

QY-x1109 人腎小球髓母細胞HBZY-1                                                  

QY-x1110 人腎小球系膜細胞HMC                                                      

QY-x1111 人腎上腺皮質(zhì)腺癌細胞NCI-295R                                                        【人B細胞活化因子受體(BAFF-R)ELISA試劑盒廠家】

QY-x1112 HEK293,人胚腎上皮細胞293A                                                    

QY-x1113 人胚腎細胞293                                                    

QY-x1114 SV40永生化的人胚腎上皮細胞293T                                                        

QY-x1118 SV40T轉(zhuǎn)化的人胚腎細胞（亞系）293T/17                                                       

QY-x1119 人類胚胎腎臟表皮細胞H293T                                                   

QY-x1120 人胚腎細胞（亞系克�。�FIP293                                               

QY-x1121 穩(wěn)定表達EBNA1的人胚腎細胞293E                                                

QY-x1122 表達SV40T和EBNA1的人胚腎細胞293ET                                              

QY-x1123 人胚腎細胞AD293                                                       

QY-x1124 Ad5DNA轉(zhuǎn)化的胚腎HEK-293                                                      

QY-x1125 人原胚腎轉(zhuǎn)化細胞系293 Ad5+                                                  

QY-x1126 表達SV40T抗原人胚腎上皮細胞293FT                                                    

QY-x1127 人人胚腎細胞AAV-293                                                

QY-x1128 人胚腎上皮包裝細胞GP2-293                                                    

QY-x1129 人胚腎上皮包裝細胞GP2-293Luc                                                       

QY-x1130 人腎上腺腺瘤細胞NCI-H205                                                        【人B細胞活化因子受體(BAFF-R)ELISA試劑盒廠家】

QY-x1131 人胚腎上皮細胞HK-2C                                               

QY-x1132 人腎癌細胞KC                                                      

QY-x1133 人胚腎上皮細胞HKC                                                   

QY-x1134 人腎小管近段上皮細胞HK-2                                                      

QY-x1135 人腎上腺神經(jīng)母細胞瘤細胞(腦轉(zhuǎn)移)KP-N-NS                                                   

9237102  Upgrade, BR plus 2 ext. devices       Upgrade BioRobot with two approved integration devices    CNY                             

9232974  Card, x-main, peristaltic XPUPG                CNY                             

9232972  YZ 96 CPU Board/Driver Unit            CNY                             

9232947  Springcontact, RoboVac, BR8000             CNY                             

9236408  Storage Platform TW I, deep well             CNY                             

9232918  Board, PCON, auto vac, BR8000               CNY                             

9236334  Green LSR                  CNY                             

9236324  Fan, LiquiChip            CNY                             

9236151  Locator Plate, 96 well,pos 2,BR RP          CNY                             

9230820  Rack for reagents, Universal (zig-zag)              CNY                             

9230718  Predilution-Rack 320x2ml Rose-80           CNY                             

9230365  Cable,Interface,flat,(Syr.)-Incub.-Read.            CNY                              【人B細胞活化因子受體(BAFF-R)ELISA試劑盒廠家】

9021284  Safety lock, waste drawer, v1, Qsym                 CNY                             

9238611  Rubber seal, centrifuge motor                  CNY                             

9238477  Tubing, OLF pump head, RapidPlate                  CNY                             

9238245  Tray for Reaction               CNY                             

9238191  Upgrade App., DirectPrep 96           CNY                             

9237721  EZ1, Full Agreement         The Full Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).   CNY                            

9240676  Bimba Cylinder, Latch Lock, AP98             CNY                             

9240668  Nut, Flangeless,1/4-28, Blue, AP                CNY                             

9240632  Power Supply, 48VDC 4.0 AMP, AP96                CNY                             

9240605  Pressure Gauge, Digital, AP196/98           CNY                             

9240602  Sensor, Sick, Waste Btl. Present, AP                  CNY                             

9240561  Control Pad, Eppendorf, AP196/98            CNY                             

9240558  Temp sensor, Bowl, Eppendorf, AP196/98                  CNY                             

9240540  Right Angle Con., SiNug, Motor, AP96/196                CNY                             

9240535  Motor Assy, SiNug, 23, Pump, AP96/196           CNY                             

9240517  Cable, Dual, USB transition, AP196/98              CNY                              【人B細胞活化因子受體(BAFF-R)ELISA試劑盒廠家】

9240195  Holder, click＇n go cassettes, HSPS             CNY                             

9240101  Re-Installation, BR MDx  The Re-Installation, BR MDx facilitates quick implementation of system back into lab routine by minimizing instrument set-up phase. It covers hardware ａnd software check of instrument ａnd provides user with operator refesher overview.  Re-installation includes labor ａnd travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.          CNY                             

9239856  Pneumatic Cylinder, v2.0          CNY