價格詳細(xì)資料：試劑盒價格電議，齊一生物銷售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認(rèn)同。齊一生物大品牌保證，傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)產(chǎn)品。

【人淋巴毒素α(LTA)ELISA試劑盒現(xiàn)貨】注意事項

1. 當(dāng)混合蛋白溶液時應(yīng)盡量輕緩，避免起泡。

2. 洗滌過程非常重要，不充分的洗滌易造成假陽性。

3. 一次加樣時間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。

4. 請每次測定的同時做標(biāo)準(zhǔn)曲線，做復(fù)孔。

5. 如標(biāo)本中待測物質(zhì)含量過高，請先稀釋后再測定，計算時請*后乘以稀釋倍數(shù)。

6. 在配制標(biāo)準(zhǔn)品、檢測溶液工作液時，請以相應(yīng)的稀釋液配制，不能混淆。

7. 底物請避光保存。

8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【人淋巴毒素α(LTA)ELISA試劑盒現(xiàn)貨】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【人淋巴毒素α(LTA)ELISA試劑盒現(xiàn)貨】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【人淋巴毒素α(LTA)ELISA試劑盒現(xiàn)貨】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

5000-1322        Cord Line 115V, SKR1                 CNY                             

5000-1318        Base Plate/Upper Body, SKR1           CNY                             

5000-1310        Gasket Rubber, SKR1                 CNY                             

5000-1255        Flat Cable CPU DETECTR, DML2                CNY                              【人淋巴毒素α(LTA)ELISA試劑盒現(xiàn)貨】

5000-1020        DML 2000 Luminometer, 240V                 CNY                             

5025-1021        Expand 4 Power Supply (Europe)               CNY                             

5000-1510        Jack Thermocouple, HTR1                 CNY                             

6000-3123        Plate Washer I - Reservoir                 CNY                             

6000-3113        Plate Washer I - Manifold                  CNY                             

EA10323  EASYartus CMV RG PCR Kit 1            CNY                             

EA10024  EASYartus HSV-1/2 RG_II           CNY                             

9240743  Belt, Centrifuge, AP96              CNY                             

5000-1414        Knob RB673STKWL Black, VTX2                CNY                             

9240699  Ring, Waste Adapator, AP                 CNY                             

5000-00031      DML 3000 120-240V                  CNY                             

9019960  careHPV test Magnetic Plate Holder                CNY                             

1050875  Elution Tubes (1,5 ml)       The bag contains 50 tubes of 1.5 ml.       CNY                             

1048146  QIAamp DNA Accessory Set B          CNY                             

280305     cador BVDV RT-PCR Kit (96)              CNY                             

289970     intype IC-RNA (1ml)          CNY                              【人淋巴毒素α(LTA)ELISA試劑盒現(xiàn)貨】

273503     pigtype Trichinella Ab (5)          CNY                             

281603     virotype SBV RT-PCR Kit (24)             CNY                             

281805     virotype CSFV RT-PCR Kit (96)           CNY                             

939013     Buffer D2 (2 x 15 ml)                  CNY                             

932156     #QIAsymphony AXpH DNA Kit (192)                  CNY                             

9001906  #QIAgility (w/o PC) Priority      QIAgility (w/o PC) Priority includes the QIAgility instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents. CNY                             

800321     Miniprep, PCR purification                CNY                             

9019346  PCB, LED w/cable, 10way connector, RG6                CNY                             

9020577  Door Sensor, Photologic, .037, DML 3000                 CNY                             

9020597  Light Plate Fixture, DML 3000                   CNY                             

9020598  Veritas Light Plate, DML 3000                   CNY                             

9013374  Tubing Set,  probe connector, BR8000           CNY                             

9013256  Tubing set, wash pump, 90/120, 0            CNY                             

QY-01900 PACAP-27/38腺苷酸環(huán)化酶激活肽-27/38（抗原）                  0.5mg/6501mg/1100                                  

QY-02000 PACAP-38腺苷酸環(huán)化酶激活肽-38（抗原）                  0.5mg/6501mg/1100                                  

QY-01970 PACAP/VIP receptor腺苷酸環(huán)化酶激活肽/血管活性腸肽受體（抗原）             0.5mg/6501mg/1100                                  【人淋巴毒素α(LTA)ELISA試劑盒現(xiàn)貨】

QY-01980 PACAP receptor-I腺苷酸環(huán)化酶激活肽受體（抗原）                   0.5mg/6501mg/1100                                  

QY-03180 PADI4 (HL-60 PAD)(Protein-arginine deiminase type IV，PADI 4，PAD I4 )關(guān)節(jié)炎相關(guān)基因                  0.5mg/6501mg/1100                                    

QY-01960 PDGF-A血小板源性生長因子-1（抗原）               0.5mg/6501mg/1100                                  

QY-01850 PDGF-B血小板源性生長因子-B（抗原）              0.5mg/6501mg/1100                                  

QY-02310 PDGF-R-A血小板源性生長因子受體-A（抗原）                    0.5mg/6501mg/1100                                  

QY-02320 PDGF-R-B血小板源性生長因子受體-B（抗原）                    0.5mg/6501mg/1100                                  

QY-02350 PMP-22(Peripheral Myelin Protein)外周髓鞘蛋白-22 多肽            0.5mg/6501mg/1100                                  

QY-01280 PI3K磷脂酰肌醇激酶（抗原）               0.5mg/6501mg/1100                                  

QY-02340 PLGF （Placenta growth factor）小鼠胎盤生長因子（多肽抗原）           0.5mg/6501mg/1100                                  

QY-02800 PLGF （Placenta growth factor）胎盤生長因子（多肽抗原）           0.5mg/6501mg/1100                                  

QY-02810 PLGF （Placenta growth factor fragment）(human)人胎盤生長因子（多肽抗原）                   0.5mg/6501mg/1100                                  

QY-01330 PP-1蛋白磷酸酯酶-1（抗原）                0.5mg/6501mg/1100                                  

QY-02500 PPAR-delta (peroxisome proliferator-activated receptor)D型-過氧化酶活化增生受體(多肽）                    0.5mg/6501mg/1100                                  

QY-00290 PP-2A （protein phosphatase 2A）蛋白質(zhì)磷酸酶-2A（抗原）            0.5mg/6501mg/1100                                  

QY-00290 PP-2A （protein phosphatase 2A）蛋白質(zhì)磷酸酶-2A（抗原）            0.5mg/6501mg/1100                                  

QY-00300 PP-2B alpha 1 蛋白磷酸酯酶-2B 催化亞單位（抗原）                0.5mg/6501mg/1100                                   【人淋巴毒素α(LTA)ELISA試劑盒現(xiàn)貨】

QY-01110 PR （Progestogerone receptor）孕激素受體（抗原）                0.5mg/6501mg/1100                                  

QY-00240 PS-1（NT），Presennillin-1（S182）早老素蛋白-1（抗原）                  0.5mg/6501mg/1100                                  

QY-00250 PS-1，Presennillin-1（CT）早老素蛋白-1（S182）                0.5mg/6501mg/1100                                  

QY-08030 BRCA1（Breast cancer susceptbility gene 1）(mouse rat)乳腺癌易感基因1抗原(小鼠 大鼠)                   0.5mg/6501mg/1100                                  

QY-0802R          BRCA1（Breast cancer susceptbility gene 1）（human）乳腺癌易感基因1抗原（人）            0.5mg/6501mg/1100                                    

QY-01790 PSD-95 突觸后密度蛋白95（抗原）            0.5mg/6501mg/1100                                  

QY-01820 PTP-1B (Protein-tyrosine phosphatase, non-receptor )蛋白酪氨酸磷酸酶-1B（抗原）                0.5mg/6501mg/1100                                  

QY-02510 RAR-α (Retinoic acid -R-α)維甲酸受體-α 多肽                  0.5mg/6501mg/1100                                  

QY-03190 glycoprotein (E1 glycoprotein) CAS:Rubella virus 風(fēng)疹病毒糖蛋白多肽片段                 0.5mg/6501mg/1100                                 

QY-01210 SAP (Synapse associated protein- I)突觸相關(guān)蛋白-1（抗原）                 0.5mg/6501mg/1100                                  

QY-01300 SARS S-protein（抗原）           0.5mg/6501mg/1100                                  

QY-01320 SARS putative orflab polyprotein （SARS coronavirus CUHK-W1）（抗原）                    0.5mg/6501mg/1100