1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【人白細(xì)胞介素-8（IL-8）ELISA試劑盒廠家】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說明！

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【人白細(xì)胞介素-8（IL-8）ELISA試劑盒廠家】齊一生物科技?xì)g迎您訂購(gòu)咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人白細(xì)胞介素-8（IL-8）ELISA試劑盒廠家】齊一生物供應(yīng)的ELISA試劑盒ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性夾心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽性. 【人白細(xì)胞介素-8（IL-8）ELISA試劑盒廠家】

以上資料僅供參考了解，不供于論文寫作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

12291       QIAfilter Plasmid Giga Kit (5)   5 QIAGEN-tip 10000, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges   CNY                            

133280     NeXtalStock TRICINE pH 7.5 (200)            CNY                             

4504005  artus Parvo B19 LC PCR Kit (96) RUO                CNY                             

9240726  Tube Assy, Jar/Pump, AP96/196                 CNY                             

9240725  Tube Assy, Fill, AP96/196           CNY                             

9240714  Contact Blade Kit, Capper, AP          CNY                             

L2172       hc2 GC-ID DNA Test PI (CD-ROM)             CNY                             

L2171       hc2 CT-ID HC2 DNA Test PI (CD-ROM)               CNY                             

12945       QIAGEN Plasmid Plus Midi Kit (100)         "100 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 100 QIAfilter Midi Cartridges

QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega ａnd Giga) or up to 24 (Midi ａnd Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction."    CNY                             

9240704  Nipple, Suction Cup, AP             CNY                             

9240689  Jam nut, Shaker Bimba, AP98           CNY                              【人白細(xì)胞介素-8（IL-8）ELISA試劑盒廠家】

330540     Supplemental Microbial qPCR MM (Fluor)                CNY                             

208252     QuantiNova Probe PCR Kit (100)               CNY                             

288107     bactotype Mycoplasma Mg/Ms PCR Kit (480)                   CNY                             

1053393  Buffer RLT Plus (220 ml)   Please use this for replacement ａnd stand-alone selling CNY                             

274302     flocktype IBV Ab (2)          CNY                             

274012     flocktype AIV Ab (2)          CNY                             

272702     pigtype PRRSV Ab (2)                 CNY                             

273805     pigtype Yersinia Ab (20)            CNY                             

871101     EGFR PCR Kit (25)              CNY                             

9002244  QIAcube(220-240v) Promo, Priority         QIAcube (220-240V) Promo Priority includes the QIAcube instrument +promo iPad, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.        CNY                             

9018909  Rotor-Disc 100 Loading Block           CNY                             

9019520  ZLV ADAPTER KIT INVITEK                CNY                             

9020574  LED Assy, Green, 15" Long, DML 3000             CNY                             

9020599  Head Alignment Fixture, DML 3000                  CNY                             

9018042  Splash Guard, RCS1           CNY                             

9018190  Tool, arm parallelism, calibration, QIAC           CNY                              【人白細(xì)胞介素-8（IL-8）ELISA試劑盒廠家】

9022614  Ipad 3                CNY                             

9013384  Board, x-main, BR8000             CNY                             

9013248  BGR System Liquid            CNY                             

9013218  ZLV Screws, plastic, M6 (20)             CNY                             

9013217  ZLV Screws, plastic, M5 (20)             CNY                             

9013205  BGR Labhand li+re Digene                CNY                             

QT-00121 地衣芽孢桿菌ATCC11946      凍干粉                                         

QT-00122 谷氨酸棒桿菌ATCC13032      凍干粉                                         

QT-00123 熏衣草灰鏈霉菌ATCC13306 凍干粉                                         

QT-00124 豬霍亂沙門氏菌豬霍亂亞種ATCC13312      凍干粉                                         

QT-00125 脫氮假單胞菌ATCC13867      凍干粉                                         

QT-00126 耐放射異常球菌ATCC13939 凍干粉                                         

QT-00127 綠針假單胞菌ATCC13985      凍干粉                                         

QT-00128 腸炎沙門氏菌腸炎亞種ATCC14028      凍干粉                                         

QT-00129 巨大芽孢桿菌ATCC14581      凍干粉                                         

QT-00130 吸水鏈霉菌奧薩霉素亞種ATCC15420 凍干粉                                         

QT-00131 長(zhǎng)雙歧桿菌ATCC15707 凍干粉                                         

QT-00132 放射性根瘤菌ATCC15955      凍干粉                                          【人白細(xì)胞介素-8（IL-8）ELISA試劑盒廠家】

QT-00133 短密青霉ATCC16024      凍干粉                                         

QT-00134 副溶血性弧菌ATCC17802      凍干粉                                         

QT-00135 單核增生李斯特菌ATCC19115      凍干粉                                         

QT-00136 缺陷短波單胞菌ATCC19146 凍干粉                                         

QT-00137 師崗鏈霉菌ATCC19166 凍干粉                                         

QT-00138 脫氮付球菌ATCC19367 凍干粉                                         

QT-00139 絲狀鏈霉菌ATCC19753 凍干粉                                         

QT-00140 裂殖壺菌ATCC20888      凍干粉                                         

QT-00141 產(chǎn)朊假絲酵母ATCC22023      凍干粉                                         

QT-00142 乙酸鈣不動(dòng)桿菌ATCC23055 凍干粉                                         

QT-00143 珊瑚鏈霉菌ATCC23901 凍干粉                                         

QT-00144 菲律賓鏈霉菌ATCC23905      凍干粉                                         

QT-00146 里氏木霉ATCC24449      凍干粉                                         

QT-00147 變異鏈球菌ATCC25175 凍干粉                                         

QT-00148 沙鏈霉菌ATCC25488      凍干粉                                         

QT-00149 酪丁酸梭菌ATCC25755 凍干粉                                          【人白細(xì)胞介素-8（IL-8）ELISA試劑盒廠家】

QT-00150 蠣灰鏈霉菌ATCC27455 凍干粉                                         

QT-00151 黃曲霉ATCC28539 凍干粉                                         

QT-00152 噬糖鹽紅菌ATCC29252 凍干粉                                         

QT-00120 痤瘡丙酸桿菌ATCC11827      凍干粉                                         

　　測(cè)定小分子量物質(zhì)常用競(jìng)爭(zhēng)法，其標(biāo)準(zhǔn)曲線中吸光度與受檢物質(zhì)的濃度呈負(fù)相關(guān)。標(biāo)準(zhǔn)曲線的形狀因試劑盒所用模式的差別而略有不同。ELISA測(cè)定的標(biāo)準(zhǔn)曲線注意圖中橫坐標(biāo)為對(duì)數(shù)關(guān)系，這更有利于測(cè)定系統(tǒng)的表達(dá)。

        講述完了以上ELISA實(shí)驗(yàn)的操作要點(diǎn)，可以發(fā)現(xiàn)其實(shí)很多步驟都并不復(fù)雜，重要的是操作的時(shí)候一定要注重細(xì)節(jié)，熟能生巧，才可以掌握ELISA實(shí)驗(yàn)的精髓所在。

133269     NeXtalStock Phosphate-citr. pH 4.2 (200)                 CNY                             

990522     Filter-Tips, 200 µl (960)            CNY                             

990396     QIAcube Modified Protocol               CNY                             

4501263  artus EBV RG PCR Kit (24) CE            CNY                             

4501205  artus EBV RG PCR Kit (96) RUO                 CNY                             

4500265  artus HSV-1/2 RG PCR Kit (96) CE               CNY                             

949008     Autopure Precipitation Soln (3800 ml)              CNY                             

335001     EpiTect Methyl qPCR Assay (200)              CNY                             

990332     Filter-Tips, 200 µl (1024)          CNY                             

939011     Buffer ATL ( 4 x 50 ml)               CNY                             

9010929  Motor, Y, with gearbox encoder                CNY                             

9010855  ZLV BGR DT-Abstreifer 4-fach Plato 7...D                  CNY                             

133246     NeXtalStock Sodium phosphate pH9.2 (200)            CNY                              【人細(xì)胞角蛋白18-M65（CK18-M65）ELISA試劑盒廠家】

330601     RT² SYBR Green FAST Mastermix (8)                 CNY                             

929604     QX Wash Buffer (40 ml)             CNY                             

9010810  Source rack (96 pos) for tubes 16mm x 1                   CNY                             

9010636  Microplate station, tall    Station for accommodating a 96-well plate (or other labware with the same base dimensions) on the worktable  CNY                             

69581       DNeasy 96 Blood & Tissue Kit (4)    For 4 x 96 DNA minipreps: 4 DNeasy 96 Plates, QIAGEN Proteinase K, Buffers, Square-Well Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Caps, 96-well Plate Registers       CNY                             

63206       DyeEx 2.0 Spin Kit (250)  250 DyeEx Spin Columns, Collection Tubes (2 ml)    CNY                             

207043     QuantiTect Whole Transcriptome Kit (25)                 CNY                             

9010253  Gripper, right, landoplasted, MTP 120             CNY                             

979101     PyroMark Q96 HS Plate (100)  For sample analysis of DNA template on MD. CNY                             

979009     PyroMark Annealing Buffer (250 ml)        For annealing of sequencing primer to DNA template.   CNY                             

62124       EZ1 DSP DNA Blood Kit (48)     CE-IVD Certified Kit for Extraction of DNA from human whole Blood. To be used in combination with EZ1 DSP Instrument (SAP 9001360) ａnd EZ1 DSP Blood Card (SAP 9017713). Available Input Volumes 350 µl, selectable elution volumes 200 µl. Processing time for 6 samples 25 Minutes.       CNY                             

301709     HiPerFect Transfection Reagent (100 ml)                  CNY                             

9002199  RGQ 6plex, PC, PrioPLUS, no PM     "RGQ 6plex, PC Instrument

Installation of RGQ 2plex & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

Loaner available in 2 days*

No preventive Maintenance

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"          CNY                             

9002196  RGQ 2plex HRM, PC, PrioPLUS, basic,no PM   "RGQ 2plex HRM, PC Instrument

Installation of RGQ 2plex HRM & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10

Loaner available in 2 days*

Preventive Maintenance (RRC,  1 PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"          CNY                             

206546     Type-it HRM PCR Kit (2000)              CNY                             

206541     Rotor-Gene Type-it HRM Discovery Kit            CNY                              【人細(xì)胞角蛋白18-M65（CK18-M65）ELISA試劑盒廠家】

9012731  Diluent Trough + lid PK 80 white               CNY                             

9012721  syringe pump inlet tubing Plato 3100                CNY                             

978803     PyroMark OneStep RT-PCR Kit (200)                 CNY                             

133231     NeXtalStock Sodium cacodyl. pH 6.8 (100)                CNY                             

QT-00020 生孢梭菌ATCC19404      凍干粉                                         

QT-00022 破傷風(fēng)桿菌/梭菌ClostridiumtetaniATCC19406     凍干粉                                         

QT-00024 化膿性鏈球菌ATCC19615      凍干粉                                         

QT-00025 黃桿菌屬ATCC21047      凍干粉                                         

QT-00026 近平滑念珠菌ATCC22019      凍干粉                                         

QT-00027 變異鏈球菌ATCC25175 凍干粉                                         

QT-00028 脆弱擬桿菌ATCC25285 凍干粉                                         

QT-00029 洋蔥假單胞菌ATCC25416      凍干粉                                         

QT-00030 大腸埃希氏菌ATCC25922      凍干粉                                         

QT-00032 金黃色葡萄球菌ATCC25923 凍干粉                                          【人細(xì)胞角蛋白18-M65（CK18-M65）ELISA試劑盒廠家】

QT-00034 宋內(nèi)氏志賀氏菌(Sh.sonnei)ATCC25931 凍干粉                                         

QT-00035 金黃色葡萄球菌ATCC27217 凍干粉                                         

QT-00037 創(chuàng)傷弧菌ATCC27562      凍干粉                                         

QT-00038 銅綠假單胞桿菌（綠膿桿菌）ATCC27853  凍干粉                                         

QT-00040 糞腸球菌ATCC29212      凍干粉                                         

QT-00041 金黃色葡萄球菌ATCC29213 凍干粉                                         

QT-00047 兩歧雙歧桿菌ATCC29521      凍干粉                                         

QT-00049 多形擬桿菌ATCC29741 凍干粉                                         

QT-00051 糞腸球菌ATCC33186      凍干粉                                         

QT-00053 空腸彎曲菌ATCC33291 凍干粉                                         

QT-00055 金黃色葡萄球菌ATCC33591 凍干粉                                         

QT-00057 大腸埃希氏菌ATCC35218      凍干粉                                         

QT-00059 屎腸球菌ATCC35667      凍干粉                                         

QT-00061 變異鏈球菌ATCC35668 凍干粉                                         

QT-00063 遲緩真桿菌/遲緩埃格特菌ATCC43055         凍干粉                                         

QT-00065 金黃色葡萄球菌ATCC43300 凍干粉                                          【人細(xì)胞角蛋白18-M65（CK18-M65）ELISA試劑盒廠家】

QT-00067 弗氏檸檬酸桿菌ATCC43864 凍干粉                                         

QT-00069 淋病柰瑟氏菌ATCC49226      凍干粉                                         

QT-00071 流感嗜血桿菌ATCC49247      凍干粉                                         

QT-00072 肺炎鏈球菌ATCC49619 凍干粉                                         

QT-00074 流感嗜血桿菌ATCC49766      凍干粉