價(jià)格詳細(xì)資料：試劑盒價(jià)格電議，齊一生物銷售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認(rèn)同。齊一生物大品牌保證，傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)產(chǎn)品。

【大鼠血漿α顆粒膜蛋白(GMP-140)ELISA試劑盒廠家】注意事項(xiàng)

1. 當(dāng)混合蛋白溶液時(shí)應(yīng)盡量輕緩，避免起泡。

2. 洗滌過程非常重要，不充分的洗滌易造成假陽性。

3. 一次加樣時(shí)間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。

4. 請(qǐng)每次測定的同時(shí)做標(biāo)準(zhǔn)曲線，做復(fù)孔。

5. 如標(biāo)本中待測物質(zhì)含量過高，請(qǐng)先稀釋后再測定，計(jì)算時(shí)請(qǐng)*后乘以稀釋倍數(shù)。

6. 在配制標(biāo)準(zhǔn)品、檢測溶液工作液時(shí)，請(qǐng)以相應(yīng)的稀釋液配制，不能混淆。

7. 底物請(qǐng)避光保存。

8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【大鼠血漿α顆粒膜蛋白(GMP-140)ELISA試劑盒廠家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【大鼠血漿α顆粒膜蛋白(GMP-140)ELISA試劑盒廠家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【大鼠血漿α顆粒膜蛋白(GMP-140)ELISA試劑盒廠家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

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19075       Buffer AL 216 ml for 1000 preps      CNY                             

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133259     NeXtalStock HEPES sod. salt pH 8.5 (200)                 CNY                             

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990380     Rack Labeling Strips, Accessory Set          CNY                             

990012     Disposable Tips, 50µl (960)               CNY                             

330621     RT² SYBR Green ROX FAST Mastermix (8)                 CNY                             

330620     RT² SYBR Green ROX  FAST Mastermix (2)              CNY                             

9010854  Motor, gripper V ａnd handler V                CNY                             

981400     Rotor-Disc OTV Kit (1)      "Kit for optical temperature verification of Rotor-Gene systems (includes a Rotor-Disc preloaded with thermochromatic liquid crystals, fluorescent inserts, CD with calibration files)

REQUIRES ROTOR-DISC 72 ROTOR AND LOCKING RING OR ROTOR-DISC 72 STARTER KIT" CNY                              【大鼠血漿α顆粒膜蛋白(GMP-140)ELISA試劑盒廠家】

133244     NeXtalStock Sodium malonate pH 6.0 (200)             CNY                             

9010363  LabHand, standard for Plato 1                   CNY                             

979105     PyroMark Q96 HS Tip Storage Box           CNY                             

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9012714  Tubing set, auto vac, BR8000           CNY                             

9012708  Peristaltic Pump Outlet Tubing 3100,4002                CNY                             

9002171  QIAcube (230V) HID Diff. Washing Station                CNY                             

929530     QX Alignment Marker 15 bp/600 bp (1.5ml)             CNY                             

971480     therascreen® EGFR Pyro Kit (24) CE                  CNY                             

133214     NeXtalStock MMT pH 4.0 (200)                 CNY                             

301702     HiPerFect Transfection Reagent (0,1ml)          CNY                             

301107     PolyFect Transfection Reagent(4 x1.0 ml)        "

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QY-12792 Anti-ET-1（Endothelin-1）內(nèi)皮素-1抗體                                                    

QY-12802 anti-ets1/E26 (E-twenty six 1) Ets轉(zhuǎn)錄因子家族ets1抗體                                                

QY-12812 Anti-Eukaryotic aspartyl protease(Rice)天冬氨酸蛋白酶家族蛋白抗體(水稻蛋白的一種)                                                      

QY-12822 Anti-EV71（Human enterovirus 71）腸道病毒71型/手足口病病毒抗體                                               

QY-12832 Anti-EXPB-2(beta-expansin)death inducer-obliterator-2植物延展素-β                                                     

QY-12842 Anti-Fabp2 (fatty acid binding protein 2, intestinal)脂肪酸結(jié)合蛋白2抗體                                                       

QY-12852 Anti-FABP(brain) （Fatty acid-binding protein brain)腦型脂肪酸結(jié)合蛋白抗體                                                       

QY-12862 Anti-factor VIII（FVIII）第八因子相關(guān)抗原抗體                                               【大鼠血漿α顆粒膜蛋白(GMP-140)ELISA試劑盒廠家】

QY-12872 Anti-factor VIII（FVIII）第八因子相關(guān)抗原抗體                                              

QY-12882 Anti-FADD (Fas-associated protein with death domain)Fas死亡結(jié)構(gòu)域相關(guān)蛋白                                                      

QY-12892 Anti-FAM3B/PANDER(PANcreatic DERived factor)胰腺衍生因子抗體                                               

QY-12902 Anti-FAS/Apo-a1/CD95(TNFR superfamily member;FasL receptor)(mouse rat)載脂蛋白A1抗體                                                       

QY-12912 Anti-Apo-a1/apoA1 (Apolipoprotein A-1; APOA1 protein )human載脂蛋白A1抗體                                                  

QY-12922 Anti-FasLAnti-FasLFasL抗體                                                 

QY-12932 Anti-FBN1 (fibrillin 1)原纖維蛋白1抗體                                                      

QY-12942 Anti-Fe65 proteinFe65 蛋白抗體                                                

QY-12952 Anti-FGF1/aFGF/ECGF-beta/HBGF-1肝素結(jié)合生長因子/酸性成纖維細(xì)胞生長因子抗體                                               

QY-12962 Anti-FGF3/Oncogene INT2(Fibroblast Growth Factor3)纖維母細(xì)胞生長因子3抗體                                                

QY-12972 Anti-FGF3/HSTF1/HBGF-4(Fibroblast Growth Factor4)纖維母細(xì)胞生長因子4抗體                                                  

QY-12982 Anti-FGF5(Fibroblast Growth Factor 4)纖維母細(xì)胞生長因子5抗體                                                 

QY-12992 Anti- KGF/FGF-7（Keratinocyte growth factor precursor)角質(zhì)形成細(xì)胞生長因子受體/纖維母細(xì)胞生長因子-7抗體                                                   

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QY-13012 anti-FGF-10(Fibroblast Growth Factor-10)成纖維細(xì)胞生長因子-10/纖維母細(xì)胞生長因子-10抗體                                               

QY-13022 Anti-KGFR（kilodalton growth factor receptors）角質(zhì)形成細(xì)胞生長因子受體抗體                                                【大鼠血漿α顆粒膜蛋白(GMP-140)ELISA試劑盒廠家】

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QY-13042 Anti-FGFR2（Fibroblast Growth Factor Receptor 2）成纖維細(xì)胞生長因子受體2抗體                                                  

QY-13052 Anti-FGFR3（Fibroblast Growth Factor Receptor 3）成纖維細(xì)胞生長因子受體3抗體                                                  

QY-13062 Anti-FGFR4（Fibroblast Growth Factor Receptor 4）成纖維細(xì)胞生長因子受體4抗體                                                  

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