【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準備試劑，樣品和標準品

2.加入準備好的樣品和標準品，37℃反應30分鐘

3.洗板5次，加入酶標試劑，37℃反應30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計算
齊一生物供應ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據(jù)。為了保證實驗數(shù)據(jù)的可靠性，在實驗過程中必須堅持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標本前都必須有一個完整的計劃。

【小鼠神經(jīng)營養(yǎng)因子4(NT-4)ELISA試劑盒價格】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【小鼠神經(jīng)營養(yǎng)因子4(NT-4)ELISA試劑盒價格】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍色）

3.動物組織勻漿（細胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標

              1g組織加入1ml組織裂解液加入8ml PBS 測細胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【小鼠神經(jīng)營養(yǎng)因子4(NT-4)ELISA試劑盒價格】樣本離心轉(zhuǎn)速及時間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應將其分成小部分-70 ℃保存，避免反復冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應在室溫下解凍并確保樣品均勻地充分解凍。

【小鼠神經(jīng)營養(yǎng)因子4(NT-4)ELISA試劑盒價格】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9241777  QIAxtractor, Inspection Service    "The Annual Inspection Service, QIAxtractor is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAextractor are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report." CNY                            【小鼠神經(jīng)營養(yǎng)因子4(NT-4)ELISA試劑盒價格】

9241375  Magnet, door lock, top, EZ1a              CNY                           

9241334  Keypad foil, EZ1           CNY                           

9240750  Belt, Elevator, AP           CNY                           

9243648  Acceler. Valid Supp, Other Extract Sys The QIAGEN Accelerated Validation Support Other Extraction System service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide provide an introduction to the instrument ａnd hands-on user support.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included by QIAGEN.  Attendees will receive a certificate of participation in the hands-on exercises.  Please indicate which protocols to validate (Biosprint 96, DNA mericon).      CNY                           

9243553  #BR M96, PM Service   "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage" CNY                           

9242864  QIArent&go -Rent out fee QSSP/AS Cabinet              CNY                           

5000-1321      Feet Set/4, SKR1           CNY                           

5000-1288      DML 2000 - Fuse 160 mA PKG 10              CNY                           

5000-1287      DML 2000 - Fuse 315 mA PKG 10              CNY                           

5000-1505      Switch ON-OFF 120V, HTR1              CNY                           

6000-00181    Plate Washer - Replacement Tubing Kit        CNY                           

6000-00178    Plate Washer - Maintenance Kit            CNY                           

5100-00047    Wash Buffer Concentrate (2 Bottles)     "2 bottles of 100mL each.

The price is proportional to 2x 5100-0795 (discontinued)"  CNY                           

9240740  Tubing, Tygon, 1/4" ID         CNY                           

9240735  Tubing, Black, Capper, AP196/98         CNY                           

9240724  Tube Assy, Draw, AP96/196         CNY                            【小鼠神經(jīng)營養(yǎng)因子4(NT-4)ELISA試劑盒價格】

5000-1412      Poteneiometer 500K, VTX2          CNY                           

9240506  Monitor, 17", Flat Panel, AP          CNY                           

9239851  Cover, LC200        CNY                           

9239565  xMAP Alignment Microspheres            CNY                           

9019942  Barrel removal tool, CX1              CNY                           

9019474  Lid, UV, polycarb, C1x          CNY                           

9238884  Stepper motor, turntable, BS96            CNY                           

9238866  BR MDx, Premium Agreement      The Premium Cover Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day). CNY                           

9238857  Basic Cover Agreement Plato3, 1yr       The Basic Cover Agreement Plato3 provides full service coverage for the Plato3 platform. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.       CNY                           

9238838  #Twister I, Basic Agreement, no PM    The Basic Cover Agreement BR Twister I provides full service coverage for the BioRobot Twister I. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Cover Agreement, the response time is 5 working days.  CNY                           

9241220  QIAxcel, Installation      "The Installation, QIAxcel is an on-site service, conducted by a QIAGEN Service Specialist. During the installation, the QIAxcel hardware is set up according to the manufacturer’s instructions.

This installation covers the handling of the QIAxcel systems hardware ａnd familiarize with the basic instrument ａnd software functions of the BioCalculator software. All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements."  CNY                           

9241195  Cable, RS232, Belkin F2N209-06-T, 1.8m           CNY                           

9241186  Sensor, interlock2, with cable, QX              CNY                           

9238831  BR 8000, Basic Agreement    The Basic Cover Agreement 8000 provides full service coverage for the BioRobot 8000. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.       CNY                           

9238822  Rect. carrier for 1 tube 100 ml             CNY                           

MADM-b       間充質(zhì)干細胞-脂肪細胞分化培養(yǎng)基-基礎 Mesenchymal Stem Cell Adipogenic Differentiation Medium-basal        500 ml                        

MADM-b-prf        間充質(zhì)干細胞-脂肪細胞分化培養(yǎng)基-無酚紅     Mesenchymal Stem Cell Adipogenic Differentiation Medium-basal-phenol red free       500ml                         

MCDM   間充質(zhì)干細胞-軟骨分化培養(yǎng)基  Mesenchymal Stem Cell Chondrogenic Differentiation Medium   500ml                         

Ared       茜素紅S染色試劑盒    Alizarin Red S Staining Kit     100 ml                        

Ored       油紅O染色試劑盒       Oil Red O Staining Kit    100 ml                        

PCR 人間質(zhì)干細胞成脂肪細胞檢測試劑盒       Human Mesenchymal Stem Cell Adipogenesis Detection Kit       50 reactions                        

HMSC-C PCR 人間充質(zhì)干細胞軟骨形成檢測試劑盒       Human Mesenchymal Stem Cell Chondrogenesis Detection Kit   50 reactions                            

SafraninO       番紅O染色試劑盒       Safranin O Staining Kit   100 reactions                            

ABlue      阿利新藍染色試劑盒    Alcian Blue Staining Kit  100 reactions                             【小鼠神經(jīng)營養(yǎng)因子4(NT-4)ELISA試劑盒價格】

HDF-f     人真皮成纖維細胞－－胎兒     Human Dermal Fibroblasts-fetal    5 x 10^5 cells/vial                       

HDF-n    人真皮成纖維細胞－－新生     Human Dermal Fibroblasts-neonate      5 x 10^5 cells/vial                       

HDF-a     人真皮成纖維細胞－－成人     Human Dermal Fibroblasts-adult   5 x 10^5 cells/vial                       

HEK-n     人表皮角質(zhì)細胞－－新生 Human Epidermal Keratinocytes-neonatal     5 x 10^5 cells/vial                       

AEpiCM  肺泡上皮細胞培養(yǎng)基    Alveolar Epithelial Cell Medium     500 ml                        

KM  角質(zhì)細胞培養(yǎng)基  Keratinocyte Medium     500 ml                        

HGL PCR       人胚層檢測試劑盒       Human Germ Layer Detection Kit  50 reactions                       

HSC PCR       人干細胞多能性檢測試劑盒       Human Stem Cell Pluripotency Detection Kit       50 reactions                       

ALP 堿性磷酸酶活性測定試劑盒       Alkaline Phosphatase Activity Assay      500 tests                            

ALPr       堿性磷酸酶染色檢測（紅色）    Alkaline Phosphatase Staining Assay (Red)   500 tests                            

EndoFectagen®     內(nèi)皮細胞轉(zhuǎn)染試劑盒   Endothelial Cell Transfection Kit    250 Transfections                      

FibroFectagen®    成纖維細胞轉(zhuǎn)染試劑   Fibroblast Transfection Kit    200 Transfections                      

EpiFectagen®       上皮細胞轉(zhuǎn)染試劑盒    Epithelial Cell Transfection Kit      100 Transfections                      

MesenFectagen®   間充質(zhì)干細胞轉(zhuǎn)染試劑盒    Mesenchymal Stem Cell Transfection Kit     250 Transfections                      

AstroFectagen®    星形膠質(zhì)細胞轉(zhuǎn)染試劑盒         Astrocyte Transfection Kit    250 Transfections                      

SMCFectagen®     平滑肌細胞轉(zhuǎn)染試劑盒      Smooth Muscle Cell Transfection Kit    250 Transfections                       【小鼠神經(jīng)營養(yǎng)因子4(NT-4)ELISA試劑盒價格】

KeratoFectagen®   角質(zhì)細胞轉(zhuǎn)染試劑盒   Keratinocyte Transfection Kit       200 Transfections                      

EpiFectagen II®     上皮細胞轉(zhuǎn)染試劑盒       Epithelial Cell Transfection Kit      250 Transfections  無貨                    

IVTFA    體外管腔形成分析試劑盒  In Vitro Tube Fomation Assay (Angiogenesis Assay)   50 tests/96 well plate                         

Col  I型膠原細胞粘附檢測試劑盒         Collagen I Cell Adhesion Assay      48 tests/48 well plate                         

Fibro       纖維粘連細胞粘附檢測試劑盒   Fibronectin Cell Adhesion Assay    48 tests/48 well plate