價格詳細資料：試劑盒價格電議，齊一生物銷售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認同。齊一生物大品牌保證，傾力為國內外科研院校實驗室提供*優(yōu)質產(chǎn)品。

【小鼠胰島素樣生長因子結合蛋白1(IGFBP-1)ELISA試劑盒廠家】注意事項

1. 當混合蛋白溶液時應盡量輕緩，避免起泡。

2. 洗滌過程非常重要，不充分的洗滌易造成假陽性。

3. 一次加樣時間控制在5分鐘內，如標本數(shù)量多，推薦使用排槍加樣。

4. 請每次測定的同時做標準曲線，做復孔。

5. 如標本中待測物質含量過高，請先稀釋后再測定，計算時請*后乘以稀釋倍數(shù)。

6. 在配制標準品、檢測溶液工作液時，請以相應的稀釋液配制，不能混淆。

7. 底物請避光保存。

8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【小鼠胰島素樣生長因子結合蛋白1(IGFBP-1)ELISA試劑盒廠家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【小鼠胰島素樣生長因子結合蛋白1(IGFBP-1)ELISA試劑盒廠家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【小鼠胰島素樣生長因子結合蛋白1(IGFBP-1)ELISA試劑盒廠家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9238977  Cartridge Rack for Reaction, V2                CNY                              【小鼠胰島素樣生長因子結合蛋白1(IGFBP-1)ELISA試劑盒廠家】

9238800  Bucket for up to 3 microplates         Sigma 13220    CNY                             

9238714  Front panel, Zent1            CNY                             

9232325  Cable, power, LSB, BR8000               CNY                             

9232150  Clamp, tip, W-drive, BR8000v1.0              CNY                             

9238907  Upper casing, BS96           CNY                             

9238905  Shaft, turntable, BS96               CNY                             

9238697  Eprom 4K15, Zent2, imbalance sensor    9238697 is a microchip that plugs into the microcontroller board which controls the imbalance sensor.    CNY                             

9020060  Casing incl. Keypad, TLyser 2             CNY                             

9014967  Z-Module 1100µl, Soldered, BR8 MDx v1.0             CNY                             

9014835  AC-Box, 400W, BR8, RD v2.0            CNY                             

9014815  RoboVac, Compete, BR8 RD v4.2    robovac complete set. includes 9014152, 9014563, 9014154 ａnd 9011275. This is the latest version-Jenith         CNY                             

9014805  PCB, SamTrack ST2CON, BR8 RD              CNY                             

9022579  PCB, X-sledge, Pipettor, DCU            CNY                             

9022497  EZ1 Adv. XL DNA Invest.L.Scale Bone Card               CNY                             

9022179  Washer Bottle Assembly, RCS           CNY                             

9022126  Tool, bearing mounting tools, RGQ          CNY                              【小鼠胰島素樣生長因子結合蛋白1(IGFBP-1)ELISA試劑盒廠家】

9021979  Lid (blue), QXA          CNY                             

9238782  Swing-out rotor 32 x 2,2 / 1,5 ml              CNY                             

9238778  BioSprint96, Full Agreement, no PM        The Full Agreement BioSprint96 provides full service coverage for the BioSprint 96. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Full Agreement, there is a priority response time of 48 hours (2 working days).       CNY                             

9238770  Tooth belt, BS96                CNY                             

9238917  LCD, BS15                  CNY                             

9238715  Front panel, Zent2            CNY                             

9232314  Cable, flat, bridge, YZ-adapter to YZ-CP           CNY                             

9238897  IR-reader, BS15                 CNY                             

9238706  Cooling aggregate T2168GK             CNY                             

9231803  Holder, block, retractable tubing, dispe           CNY                             

9238687  Front panel, Zent3            CNY                             

9231132  Flat Band Cable 20P 1190mm Xmain-Xadp               CNY                             

9020065  Plastic hood, TLyser 2                CNY                             

9020062  Brake 205V DC, TLyser              CNY                             

9020055  Motor 100-115V 50/60 Hz, TLyser            CNY                             

HDF-f-mt 人真皮成纖維細胞-胎兒C處理    Human Dermal Fibroblasts-Fetal-Mitomycin C treated  1 x 10^6 cells/vial                              

HDF-f-mt 人真皮成纖維細胞-胎兒C處理    Human Dermal Fibroblasts-Fetal-Mitomycin C treated  2 x 10^6 cells/vial                             

HDF-f-mt 人真皮成纖維細胞-胎兒C處理    Human Dermal Fibroblasts-Fetal-Mitomycin C treated  5 x 10^6 cells/vial                               

HDF-f-mt 人真皮成纖維細胞-胎兒C處理    Human Dermal Fibroblasts-Fetal-Mitomycin C treated  1 x 10^6 cells/vial x 5                                   

HHDPC     人毛乳頭細胞         Human Hair Dermal Papilla Cells     5 x 10^5 cells/vial                                

HHGMC   人生發(fā)基質細胞     Human Hair Germinal Matrix Cells 5 x 10^5 cells/vial                                

HHORSC   人毛囊外根鞘細胞         Human Hair Follicular Outer Root Sheath Cells        5 x 10^5 cells/vial                                 【小鼠胰島素樣生長因子結合蛋白1(IGFBP-1)ELISA試劑盒廠家】

HHIRSC    人毛囊內根鞘細胞         Human Hair Follicular Inner Root Sheath Cells         5 x 10^5 cells/vial                                

HHFK        人毛囊角質細胞     Human Hair Folliclar Keratinocytes 5 x 10^5 cells/vial                                

HLEC         人淋巴內皮細胞     Human Lymphatic Endothelial Cells          5 x 10^5 cells/vial                                

HLF  人淋巴成纖維細胞         Human Lymphatic Fibroblasts 5 x 10^5 cells/vial                                

HTEpiC     人扁桃體上皮細胞         Human Tonsil Epithelial Cells   5 x 10^5 cells/vial     無貨                           

HTF  人扁桃體成纖維細胞     "Human Tonsil Fibroblasts

"       5 x 10^5 cells/vial                              

HTyF          人胸腺成纖維細胞         Human Thymus Fibroblasts      5 x 10^5 cells/vial                              

HOMEC    人口腔微血管內皮細胞         Human Oral Microvascular Endothelial Cells            無貨                           

HOK 人口腔角質細胞     Human Oral Keratinocytes       5 x 10^5 cells/vial                                

HGF 人牙齦成纖維細胞         Human Gingival Fibroblasts     5 x 10^5 cells/vial                                

HPLF         人牙周膜成纖維細胞     Human Periodontal Ligament Fibroblasts        5 x 10^5 cells/vial                                

HEEpiC     人食道微血管內皮細胞         Human Esophageal Microvascular Endothelial  Cells    5 x 10^5 cells/vial                                

HESMC     人食道平滑肌細胞         Human Esophageal Smooth Muscle Cells         5 x 10^5 cells/vial                                

HEEpiC     人食管上皮細胞     Human Esophageal Epithelial Cells  5 x 10^5 cells/vial                                

HEF  人食管成纖維細胞         Human Esophageal Fibroblasts        5 x 10^5 cells/vial                                

HGSMC    人胃平滑肌細胞     Human Gastric Smooth Muscle Cells        5 x 10^5 cells/vial                                 【小鼠胰島素樣生長因子結合蛋白1(IGFBP-1)ELISA試劑盒廠家】

HGEpiC     人胃上皮細胞         Human Gastric Epithelial Cells                   無貨                           

HIMEC      人腸微血管內皮細胞     Human Intestinal Microvascular Endothelial Cells  5 x 10^5 cells/vial                                

HISMC      人腸平滑肌細胞     Human Intestinal Smooth Muscle Cells   5 x 10^5 cells/vial                                

HIF   人腸道成纖維細胞         Human Intestinal Fibroblasts  5 x 10^5 cells/vial                                

HCoMEC  人結腸微血管內皮細胞         Human Colonic Microvascular Endothelial Cells       5 x 10^5 cells/vial                                

HCSMC     人結腸平滑肌細胞         Human Colonic Smooth Muscle Cells        5 x 10^5 cells/vial                                

HCoEpiC   人結腸上皮細胞     Human Colonic Epithelial Cells          5 x 10^5 cells/vial