1.購買我公司任意ELISA試劑盒指標(biāo)享受免費代測，并提供技術(shù)指導(dǎo)服務(wù)！

二、實驗材料與試劑配制：

1. 儀器與材料：酶標(biāo)儀（使用前預(yù)熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時備用（如果標(biāo)本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【小鼠Toll樣受體9(TLR-9/CD289)ELISA試劑盒廠家】三. 仔細(xì)閱讀說明書

1.檢查試劑盒標(biāo)簽上的有效期。如果超過有效期，請勿使用。

2. 按說明書確定所有試劑齊全（數(shù)量、體積）

3. 標(biāo)本制備要規(guī)范，每份標(biāo)本體積要按2-3個復(fù)孔以上的量制備（貯存），盡量分裝做備份。

4.短期內(nèi)無法實驗者，注意低溫保存

5.準(zhǔn)備好所有實驗額外所需物品，（比如移液器，試管，清洗器，酶標(biāo)儀）

6. 按說明書將所用試劑平衡至室溫，根據(jù)檢測標(biāo)本數(shù)量確定所需試劑的量

7.檢查試劑盒內(nèi)每種試劑的貯存條件，保證所有試劑均按照試劑盒內(nèi)說明書推薦的條件存儲。 【小鼠Toll樣受體9(TLR-9/CD289)ELISA試劑盒廠家】

8.檢查不穩(wěn)定或者變質(zhì)的試劑溶液（比如沉淀或變色）。有些試劑，比如標(biāo)準(zhǔn)品稀釋液或者檢測稀釋液，可能在設(shè)計時就含有沉淀物。所有試劑在滴入酶標(biāo)板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標(biāo)板之前，必需不停攪拌。

9.保證充分的孵育時間和溫度。

10.切勿使用不同生產(chǎn)批號的試劑取代現(xiàn)有試劑或混合現(xiàn)有試劑。

11.在混合或溶解蛋白溶液時，避免泡沫產(chǎn)生。

12.在實驗開始之前，安排好實驗流程。在實驗開始之前，清潔工作臺。

13.若有問題，應(yīng)與我公司或代理商的技術(shù)支持聯(lián)系

【小鼠Toll樣受體9(TLR-9/CD289)ELISA試劑盒廠家】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標(biāo)本前都必須有一個完整的計劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測，對收集后當(dāng)天就進(jìn)行檢測的標(biāo)本，及時儲存在4℃?zhèn)溆茫缬刑厥庠蛐枰芷谑占瘶?biāo)本，請造模取材后，將標(biāo)本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實驗質(zhì)量，所以避免反復(fù)凍融。部分標(biāo)本也可加入適當(dāng)防腐劑，激素類標(biāo)本需添加抑肽酶。 

2.試劑盒代測標(biāo)本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項請與我司技術(shù)人員溝通。

3.液體類標(biāo)本：標(biāo)本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。

4.購買前請?zhí)崆案嬖V我們：您樣本的種屬、樣本數(shù)量、待測指標(biāo)及其它特殊要求。

【小鼠Toll樣受體9(TLR-9/CD289)ELISA試劑盒廠家】五、代測數(shù)據(jù)詳細(xì)內(nèi)容

1、ELISA標(biāo)準(zhǔn)曲線；

2、實驗具體詳細(xì)步驟；

3、完整的實驗報告（試劑耗材，實驗方法，實驗結(jié)果及結(jié)果說明）；

4、實驗進(jìn)行階段，我公司客服人員會及時向您匯報實驗進(jìn)程。

9239498  V-Housing, LX100              CNY                              【小鼠Toll樣受體9(TLR-9/CD289)ELISA試劑盒廠家】

9239490  EX Laser Driver, Uniphase                  CNY                             

9239390  PCR Plate Adapter, M96           CNY                             

9236510  BR 3000, PM Service        The Overhaul covers all travel ａnd labor costs plus all necessary parts to perform a Preventative Maintenance,. The instrument will also be thoroughly checked ａnd inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed.     CNY                             

9232764  Disposable Troughs, 20 / 30 ml (10)          Troughs holding up to 20 ml of liquid; pack of 10     CNY                             

9236472  BR 9600, Full Agreement         The Full Agreement 9600 provides full service coverage for the BioRobot 9600. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).  CNY                             

9236443  BR, Installation         The Installation, BR Universal facilitates the quick implementation of the BioRobot into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the BioRobot Universal instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.         CNY                             

9239155  Assembly, vertical axis, TWI              CNY                             

9239108  Assy, Sample Needle, Actuator linkage             CNY                             

9232693  MTP-Plate Holder  2.01                   CNY                             

9232629  Bottle, 500-ml, yellow fitting            CNY                             

9232602  Vacuum manifold, plate holder bottomless              CNY                             

9232579  Cooling block, 1,5ml                   CNY                             

9238951  Locking lever, BS15           CNY                             

9238798  Rectangular bucket, sealable with cap             CNY                             

9238794  Angle rotor 6 x 250 ml               CNY                             

9016913  Tubing, press relief valve inlet DISU v2             CNY                              【小鼠Toll樣受體9(TLR-9/CD289)ELISA試劑盒廠家】

9238942  Keyboard-Display assembly, BS15             CNY                             

9238753  Screwdriver, hex2mm, z-drive BR8          CNY                             

9238916  Power supply 40W, BS96          CNY                             

9238748  Coupling, CPC, screw, male, V2                 CNY                             

9238716  Seal for lid, 1350 mm                 CNY                             

9238898  Front panel, BS15              CNY                             

9238699  Frontpanel w. keyboard foil (Zent1)                   CNY                             

9231793  Motor, gripper, handler, BR8000              CNY                             

9238690  Computer board Zent2, no Eprom            CNY                             

9231434  Probe, 0,4mm, ceramic coated for PK80                   CNY                             

9020071  Keypad, TLyser                   CNY                             

9020070  Controller 100-120V/200-240V, TLyser              CNY                             

9014996  Shaker, Highspeed Unit, BR8 RD               CNY                             

9014870  X-Motor, BR8            CNY                             

9014790  Tip Disposal Bags MDx    Autoclavable bags for collection ａnd disposal of used disposable tips, pack of 15   CNY                            

QR20-110         Rosetta(DE3)感受態(tài)細(xì)胞        10 x 100μl                                           

QR20-111         Rosetta(DE3)感受態(tài)細(xì)胞        20 x 100μl                                           

QR20-112         BCA蛋白定量試劑盒     200次                                           【小鼠Toll樣受體9(TLR-9/CD289)ELISA試劑盒廠家】

QR20-113         蛋白標(biāo)準(zhǔn)溶液-BSA 5x1ml                                            

QR20-114         SDS-PAGE凝膠制備試劑盒    1 kit                                      

QR20-115         ProteinShow-G250蛋白快速染色試劑 250ml                                           

QR20-116         30%丙烯酰胺-甲叉雙丙烯酰胺溶液(29:1)   100ml                                           

QR20-117         SDS-PAGE濃縮膠緩沖液(4x)  50ml                                              

QR20-118         SDS-PAGE濃縮膠緩沖液(4x)  200ml                                           

QR20-119         SDS-PAGE分離膠緩沖液(4x)  100ml                                           

QR20-120         SDS-PAGE分離膠緩沖液(4x)  500ml                                           

QR20-121         SDS-PAGE Loading Buffer (變性，5x)      5ml                                       

QR20-122         SDS-PAGE Loading Buffer (非變性，5x) 5ml                                       

QR20-123         10%SDS溶液   100ml                                           

QR20-124         PBS  500ml                                           

QR20-125         PBST         500ml                                           

QR20-126         TBS  500ml                                           

QR20-127         TBST         500ml                                           

QR20-128         Tris-Glycine       500ml                                            【小鼠Toll樣受體9(TLR-9/CD289)ELISA試劑盒廠家】

QR20-129         Tris-Glycine SDS        500ml                                           

QR20-130         1.5M Tris-Cl(pH8.8) 500ml                                           

QR20-131         0.5M Tris-Cl(pH6.8) 500ml                                           

QR20-132         超純dNTP(2.5mM each)         0.5ml                                            

QR20-133         超純dNTP(2.5mM each)         1ml                                       

QR20-134         超純dNTP(10mM each）       250μl                                          

QR20-135         超純dNTP(10mM each）       500μl                                          

QR20-136         超純dATP(100mM)          0.5ml                                            

QR20-137         超純dGTP(100mM)         0.5ml                                            

QR20-138         超純dCTP(100mM)          0.5ml                                            

QR20-139         超純dTTP(100mM)          0.5ml