1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【小鼠E選擇素(E-Selectin/CD62E)ELISA試劑盒價(jià)格】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買(mǎi)說(shuō)明！

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見(jiàn)的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問(wèn)世以來(lái),得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類(lèi)戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【小鼠E選擇素(E-Selectin/CD62E)ELISA試劑盒價(jià)格】齊一生物科技?xì)g迎您訂購(gòu)咨詢，齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【小鼠E選擇素(E-Selectin/CD62E)ELISA試劑盒價(jià)格】齊一生物供應(yīng)的ELISA試劑盒ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過(guò)的HRP.再經(jīng)過(guò)溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開(kāi)優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過(guò)各種化學(xué)合成方法制備.近年來(lái),隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說(shuō)（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性?shī)A心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來(lái)自于該毒株的開(kāi)放閱讀框2和開(kāi)放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過(guò)氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽(yáng)性. 【小鼠E選擇素(E-Selectin/CD62E)ELISA試劑盒價(jià)格】

以上資料僅供參考了解，不供于論文寫(xiě)作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

9240526  Cable, Motor Axis #3, Capper I/O, AP98           CNY                              【小鼠E選擇素(E-Selectin/CD62E)ELISA試劑盒價(jià)格】

9240500  Fluidic Valve, Reagent Pump, AP               CNY                             

9240240  Collet nut, TR            CNY                             

9240099  #Re-Installation, BR M48         The Re-Installation, BR M48 facilitates quick implementation of system back into lab routine by minimizing  instrument set-up phase. It covers hardware ａnd software check of instrument ａnd provides user with operator refesher overview.  Re-installation includes labor ａnd travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.          CNY                             

9239855  Compressor, v2.0               CNY                             

9019494  Measurement Gauge Kit, RCS                   CNY                             

9238863  LiquiChip, Premium Agreement       The Premium Cover Agreement LiquiChip provides full service coverage for the LiquiChip workstation. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).     CNY                             

5000-1251        Motor Control PCB, DML2                 CNY                             

5000-1513        H-Spring, DML2                 CNY                             

6000-3119        Plate Washer I - Rinse Cap Assembly                 CNY                             

5000-1417        Overlay 099A, VTX2                   CNY                             

5050-1078        LIS Information Package           CNY                             

9240717  Lead Screw Assy, Axis 5, TR, AP                 CNY                             

9240703  O-Ring, Valve, Reagent Pump, AP            CNY                             

9020806  Rotor Timing Calibration Dye, RGQ                   CNY                             

338134     Microbial DNA Positive Control                 CNY                             

288105     bactotype Mycoplasma Mg/Ms PCR Kit (96)            CNY                              【小鼠E選擇素(E-Selectin/CD62E)ELISA試劑盒價(jià)格】

275002     flocktype NDV Ab (2)                 CNY                             

285515     cador C. burnetii PCR Reagent (96)          CNY                             

285115     cador TKP PCR Reagent (96)             CNY                             

9001901  #QIAgility (PC) Priority     QIAgility (PC) Priority includes the QIAgility instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.      CNY                             

800331     PCR Amplification              CNY                             

9019489  ZLV PLATE ADAPTOR BIOHIT            CNY                             

9019544  ZLV Skirt, low raw white 2427, CASx                 CNY                             

9019580  ZLV Reed Switch assembly, C34                 CNY                             

9020583  X-Belt, DML3000 185 Teeth              CNY                             

9020592  Optical Head Assembly, DML 3000           CNY                             

9021015  Encoder, Z Axis, VR, DCU                   CNY                             

9013209  Cabling kit, 5V/24V, BR8000              CNY                             

9013189  Wheel, retractable tubing, dispenser, BR                 CNY                             

9011757  Reagent holder, 1-trough 20 ml       Holder for accommodating a single disposable trough (20 ml) on the worktable; must be used with the reagent holder tray  CNY                             

132632     NeXtal Stock Potassium formate (50)      10M Potassium formate - 50mL (4 x 12,5 ml tube) CNY                             

QTR-1000         狂犬病抗原檢測(cè)試劑盒                                                       

QTR-1001         FELINE specialties貓病                                                 

QTR-1002         貓冠狀病毒抗體檢測(cè)試劑盒                                                        【小鼠E選擇素(E-Selectin/CD62E)ELISA試劑盒價(jià)格】

QTR-1003         貓白血病抗體檢測(cè)試劑盒                                                  

QTR-1004         貓白血病病毒抗原檢測(cè)試紙條                                                  

QTR-1005         貓白血病抗原p27定量檢測(cè)試劑盒                                                   

QTR-1006         "貓免疫缺陷病(Feline

Immunodefciency (FIV))

"                                                     

QTR-1007         貓免疫缺陷病抗體檢測(cè)試劑盒                                                  

QTR-1008         貓免疫缺陷病抗體檢測(cè)試紙條                                                  

QTR-1009         貓免疫缺陷病抗體定量檢測(cè)試劑盒                                                  

QTR-1010         布魯氏菌PCR測(cè)試劑盒         24次                                             

QTR-1011         副結(jié)核分枝桿菌PCR檢測(cè)試劑盒         100次                                          

QTR-1012         支原體PCR檢測(cè)試劑盒         50次                                             

QTR-1013         瘟病毒PCR法檢測(cè)試劑盒     100次                                          

QTR-1014         偽狂犬PCR檢測(cè)試劑盒         100次                                          

QTR-1015         非洲豬瘟PCR檢測(cè)試劑盒     100次                                          

QTR-1016         豬布病PCR檢測(cè)試劑盒         50次                                             

QTR-1017         螺旋體PCR檢測(cè)試劑盒         50次                                             

QTR-1018         胞內(nèi)勞森菌PCR檢測(cè)試劑盒         50次                                              【小鼠E選擇素(E-Selectin/CD62E)ELISA試劑盒價(jià)格】

QTR-1019         胞內(nèi)勞森菌PCR檢測(cè)試劑盒         100次                                          

QTR-1020         豬圓環(huán)PCR檢測(cè)試劑盒         100次                                          

QTR-1021         豬細(xì)小病毒PCR試劑盒         100次                                          

QTR-1022         豬胃腸炎/呼吸道冠狀病毒PCR試劑盒        50次                                             

QTR-1023         豬藍(lán)耳病毒PCR試劑盒         50次                                             

QTR-1024         豬藍(lán)耳-圓環(huán)二聯(lián)PCR試劑盒        50次                                             

QTR-1025         牛傳染性鼻氣管炎PCR試劑盒100次                                                       

QTR-1026         彎曲桿菌PCR試劑盒     100次                                          

QTR-1027         傳染性法氏囊PCR試劑盒     100次                                          

QTR-1028         傳染性支氣管PCR試劑盒     100次                                          

QTR-1029         傳染性喉氣管炎PCR試劑盒         50次