選擇齊一生物的原因：

1.供貨及時(shí)。我們訂貨周期短，運(yùn)輸時(shí)間短，在*短時(shí)間內(nèi)為您提供所需產(chǎn)品。

2.價(jià)格優(yōu)惠：美國ATCC的中國代理商，沒有中間環(huán)節(jié)。在國內(nèi)我們保證價(jià)格。

3.服務(wù)保障：我們擁有專用的技術(shù)指導(dǎo)團(tuán)隊(duì)，對(duì)每售出的一件產(chǎn)品，提供全面的售前、售中和售后服務(wù)。

4.產(chǎn)品種類齊全：25個(gè)系統(tǒng)共150多種細(xì)胞以及各類菌株，滿足不同客戶的要求。細(xì)胞經(jīng)過嚴(yán)格的質(zhì)控，純度可達(dá)98％。常用產(chǎn)品備有現(xiàn)貨。其產(chǎn)品類型如下：消化系統(tǒng)細(xì)胞株，呼吸系統(tǒng)細(xì)胞株，泌尿系統(tǒng)細(xì)胞株生殖系統(tǒng)細(xì)胞株 血液循環(huán)系統(tǒng) 內(nèi)皮細(xì)胞細(xì)胞株 神經(jīng)系統(tǒng)及其他細(xì)胞株，動(dòng)物細(xì)胞株和菌株

5.對(duì)于部分難培養(yǎng)的細(xì)胞我公司可代復(fù)蘇細(xì)胞，并提供技術(shù)支持。

6.經(jīng)驗(yàn)豐富：我公司是國內(nèi)專業(yè)化的細(xì)胞銷售公司，具有十余年細(xì)胞產(chǎn)品銷售經(jīng)驗(yàn)，有專業(yè)的銷售團(tuán)隊(duì)和技術(shù)人員。齊一生物公司的每一位員工都將竭誠為您服務(wù)。

7.質(zhì)量保證，保證售后

8. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【3T3L1小鼠胚胎成纖維細(xì)胞(前脂肪)】 齊一生物公司專業(yè)代理ScienCell簡介ScienCell Research Laboratories (ScienCell) is a biotechnology company whose mission is the research ａnd development of cell ａnd cell-related products for experimental ａnd therapeutic use. The ScienCell technology provides a variety of high quality normal human ａnd animal cells, cell culture media ａnd reagents, medium supplements, cell-derived RNA, cDNA ａnd proteins for the research community. Scientists at ScienCell are also studying ａnd developing cell therapeutic strategies that will significantly improve the quality of life by achieving the cell＇s ability to combat human degenerative disorders. We are based in San Diego, California ａnd were established in 1999.

【3T3L1小鼠胚胎成纖維細(xì)胞(前脂肪)】美國ATCC簡介：美國菌種保藏中心， 又稱美國模式菌種收集中心(ATCC)，是位于馬里蘭洲洛克菲勒的一家私營的，非贏利性組織。目前它可以提供各種動(dòng)植物細(xì)胞、標(biāo)準(zhǔn)品、菌株達(dá)兩萬多種。ATCC成立于1925年，是世界上的生物資源中心，由美國14家生化、醫(yī)學(xué)類行業(yè)協(xié)會(huì)組成的理事會(huì)負(fù)責(zé)管理，是一家全球性、非盈利生物標(biāo)準(zhǔn)品資源中心。ATCC向全球發(fā)布其獲取、鑒定、保存及開發(fā)的生物標(biāo)準(zhǔn)品，推動(dòng)科學(xué)研究的驗(yàn)證、應(yīng)用及進(jìn)步 ATCC可以提供以下類別生物標(biāo)準(zhǔn)品：細(xì)胞株（3000多種）；菌株（15000多種）；動(dòng)植物病毒株（2500多種）以及重組物質(zhì)等。

現(xiàn)已成為可信賴的活體微生物、細(xì)胞系等獲得、保存和發(fā)放的國家資源中心。ATCC有29,000多種不同品系可靠的動(dòng)物細(xì)胞和微生物培養(yǎng)體，它能滿足各科學(xué)團(tuán)體對(duì)可信賴品系的需要。僅1981年就有39,000多培養(yǎng)物發(fā)放給全世界的科學(xué)工作者，并成功地用冷凍和冷凍干燥的方法保存各種細(xì)胞品系。 ATCC是一個(gè)非(學(xué)會(huì)單位)無利潤的公司，由各科學(xué)組織推選成員組成理事會(huì)進(jìn)行管理�，F(xiàn)有工作人員125人。參加的科學(xué)團(tuán)體有：美國免疫協(xié)會(huì)、美國生物化學(xué)會(huì)、美國細(xì)胞生物學(xué)會(huì)、美國微生物學(xué)會(huì)、美國動(dòng)物學(xué)會(huì)、美國遺傳學(xué)會(huì)、原生動(dòng)物學(xué)會(huì)和組織培養(yǎng)協(xié)會(huì)等16個(gè)學(xué)術(shù)組織。在ATCC可利用各種材料以及工具和設(shè)備進(jìn)行廣泛的研究。主要研究項(xiàng)目有：酶聯(lián)免疫吸附分析技術(shù)(ELISA)，真核細(xì)胞轉(zhuǎn)錄的調(diào)控，雜交瘤、淋巴類和髓類以及其他細(xì)胞系的建立，人突變細(xì)胞系的基因蛋白質(zhì)，借助計(jì)算機(jī)進(jìn)行鑒別分析，培養(yǎng)物的特征鑒別和保存技術(shù)的改進(jìn),關(guān)于化學(xué)致癌、致畸和致突變的。

【3T3L1小鼠胚胎成纖維細(xì)胞(前脂肪)】齊一生物提醒接收到細(xì)胞注意事項(xiàng)

1. 接收到細(xì)胞，肉眼觀察細(xì)胞培養(yǎng)基顏色，顯微鏡觀察細(xì)胞生長情況，并對(duì)細(xì)胞進(jìn)行不同倍數(shù)拍照（建議收到時(shí)的培養(yǎng)瓶拍一張照片，顯微鏡拍收到時(shí)的細(xì)胞100X,200X各一張）。

2.用75%的酒精消毒（建議配置75%的酒精的水是滅菌過的）。

3.嚴(yán)格無菌操作，打開細(xì)胞培養(yǎng)瓶。

4.將細(xì)胞培養(yǎng)瓶內(nèi)的培養(yǎng)基用吸管完全吸出（嚴(yán)禁直接傾倒），放入另一無菌容器中（建議換新的培養(yǎng)基培養(yǎng)細(xì)胞）。

5.用PBS 2-3ml/次輕輕洗細(xì)胞表面，洗3-4次遍，加入0.05%胰酶-EDTA1-1.5ml,顯微鏡下觀察細(xì)胞變圓，輕輕拍打培養(yǎng)瓶直到細(xì)胞脫落，加入終止液終止。

6.1000rpm，5min離心，重懸細(xì)胞。

7.換新的細(xì)胞培養(yǎng)瓶，37℃，5%CO2培養(yǎng)。【3T3L1小鼠胚胎成纖維細(xì)胞(前脂肪)】

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

201445Taq PCR Master Mix Kit (1000 Units)12 x 1.7 ml Taq PCR Master Mix‡ containing 1000 units Taq DNA Polymerase total, 12 x 1.7 ml distilled waterCNY

200205TopTaq DNA Polymerase (1000)"4 x 250U TopTaq DNA Polymerase, 10x TopTaq PCR Buffer, 1x CoralLoad Concentrate, 5x Q-solutions, 25mM MgCl2

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32561EasyXpress Insect Kit II (5)"Kit for cell-free expression of proteins in cell-free insect cell extracts from DNA templates (PCR prodcuts or plasmid with T7 promotor) 

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30430Ni-NTA Superflow (100 ml)100 ml nickel-charged resin (max. pressure: 140 psi)CNY

30310NTA Agarose (25ml)25 ml uncharged resin (max. pressure 2.8 psi)CNY

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9241177Power switch, 6A, 250VAC, QXCNY

9241147Transport  lock, X axis, M48CNY

9241032Tube insert, 2.0 ml,samp.carr., QsymCNY

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