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大鼠水通道蛋白1(AQP-1)ELISA試劑盒
點(diǎn)擊次數(shù):15發(fā)布時(shí)間:2016/6/4 18:42:45

更新日期:2016/9/30 10:32:46
所 在 地:中國大陸
產(chǎn)品型號(hào):
優(yōu)質(zhì)供應(yīng)
詳細(xì)內(nèi)容
【友情提示】:本產(chǎn)品僅供科研研究使用,不得用于人體臨床直接檢測。避免給您帶來不必要的損失,請(qǐng)仔細(xì)閱讀購買說明!
【大鼠水通道蛋白1(AQP-1)ELISA試劑盒】酶法檢測試驗(yàn)所需自備物品:
1. 酶標(biāo)儀(450nm 波長濾光片)
2. 高精度移液器,EP 管及一次性吸頭
3. 37℃恒溫箱,雙蒸水或去離子水
4. 吸水紙
試劑盒安全性提示:
1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請(qǐng)盡快用水沖洗。
2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。
3.不要用嘴吸取試劑盒里的任何成份。
【大鼠水通道蛋白1(AQP-1)ELISA試劑盒】操作注意事項(xiàng):
1.試劑盒應(yīng)按標(biāo)簽說明書儲(chǔ)存,使用前恢復(fù)到室溫30分鐘后方可使用。稀稀過后的標(biāo)準(zhǔn)品應(yīng)丟棄,不可保存。
2.實(shí)驗(yàn)中不用的板條應(yīng)立即放回包裝袋中,密封保存,以免變質(zhì)。
3.不用的其它試劑應(yīng)包裝好或蓋好。不同批號(hào)的試劑不要混用.請(qǐng)?jiān)谠噭┖斜Y|(zhì)前使用。
4.使用一次性的吸頭以免交叉污染,吸取終止液和底物A、B液時(shí),避免使用帶金屬部分的加樣器。
5.使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。
6.洗滌酶標(biāo)板時(shí)應(yīng)充分拍干,不要將吸水紙直接放入酶標(biāo)反應(yīng)孔中吸水。
7.底物A應(yīng)揮發(fā),避免長時(shí)間打開蓋子。底物B對(duì)光敏感,避免長時(shí)間暴露于光下。避免用手接觸,有毒。實(shí)驗(yàn)完成后應(yīng)立即讀取OD值。
8.加入試劑的順序應(yīng)一致,以保證所有反應(yīng)板孔溫育的時(shí)間一樣。
9.按照說明書中標(biāo)明的時(shí)間、加液的量及順序進(jìn)行溫育操作。
【大鼠水通道蛋白1(AQP-1)ELISA試劑盒】齊一生物客戶寄標(biāo)本時(shí)需注明以下情況:
1、標(biāo)本編號(hào);2、所測項(xiàng)目;3、是否做復(fù)孔;3、聯(lián)系方式;4、實(shí)驗(yàn)后標(biāo)本是否寄回。
客戶必知:
客戶應(yīng)對(duì)所提供的材料及信息負(fù)責(zé),如因客戶提供的材料及信息不準(zhǔn)確而引起的實(shí)驗(yàn)延誤或經(jīng)濟(jì)損失由客戶承擔(dān)。
齊一生物供應(yīng)ELISA試劑盒檢測的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性,在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。
樣本保存條件:
1.2-8℃冰箱*多放2周
2.-20到-50℃的冰箱可以放3-6個(gè)月
3.-50℃以下的冰箱,可以放一年
樣本收集
1.血清樣本用干燥管(紅色)或者促凝管(黃色)收集
2.血漿用抗凝管,一般有EDTA抗凝(紫色)、枸櫞酸鈉抗凝(黑色或者藍(lán)色)
3.動(dòng)物組織勻漿(細(xì)胞):
1g組織加入9ml PBS(濃度為0.1M,pH為7.4的)測分泌型指標(biāo)
1g組織加入1ml組織裂解液加入8ml PBS 測細(xì)胞內(nèi)
糞便樣本:1g樣本+9ml PBS(濃度為0.01M,pH為7.4的)
沒有PBS的話也可以用一下生理鹽水,還是用PBS
樣本離心轉(zhuǎn)速及時(shí)間
1.試管采集的樣本:3000轉(zhuǎn),離心五分鐘取上清
2.離心管或者EP管采集的樣本:5000--8000轉(zhuǎn)3分鐘取上清
3..樣本保存--如果樣品不立即使用,應(yīng)將其分成小部分-70 ℃保存,避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒,檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。
【大鼠水通道蛋白1(AQP-1)ELISA試劑盒】齊一生物供應(yīng),以上資料供參考如有疑問需要詳細(xì)資料請(qǐng)聯(lián)系我們客服人員,齊一生物銷售:021-6034 8496;181214 53965;173021 04490
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