色譜的免疫球蛋白是從非免疫的動(dòng)物血清中提取純化出來(lái)的，因此這些純化的免疫球蛋白不包含針對(duì)目已知免疫原的任何抗體.色譜的制備過(guò)程設(shè)計(jì)各種方法，包括離子交換、凝膠過(guò)濾、脫水化、金屬親和、蛋白A和其他免疫親和層析技術(shù).同時(shí)，還通過(guò)進(jìn)步的酶切消化和制備出F（ab’）2和Fab片段化抗體，這種片段化的產(chǎn)品純度J高，不含整個(gè)分子或者其他無(wú)關(guān)的片段，這些色譜的免疫球蛋白J其片段可被用作實(shí)驗(yàn)對(duì)照.

艾美捷色譜人IgM（骨髓瘤）完整分子純度J高，尤其適合用于對(duì)照分析.其中這種片段化的產(chǎn)品純度J高，不含整個(gè)分子或者其他無(wú)關(guān)的片段，這些色譜的免疫球蛋白J其片段可被用作實(shí)驗(yàn)對(duì)照.

艾美捷色譜人IgM（骨髓瘤）完整分子基本參數(shù)：

物理狀態(tài)：無(wú)菌過(guò)濾液體

儲(chǔ)存：在無(wú)菌條件下儲(chǔ)存在 2-8°C。在使用當(dāng)天準(zhǔn)備工作稀釋液。

有效期：年。如果測(cè)試結(jié)果對(duì)預(yù)期用途是可接受的，則有效期可能會(huì)延長(zhǎng)。

純度： 基于抗原濃度為 20 mg/ml 的免疫電泳，針對(duì)山羊抗人全血清的沉淀模式與針對(duì)山羊抗人 IgM、Fc 5μ片段特異性的沉淀模式相同。未檢測(cè)到針對(duì)山羊抗人 IgG、Fc 片段特異性的沉淀素線；山羊抗人 IgA，鏈特異性；或山羊抗人α-2巨球蛋白。

緩沖液： 0.01M 磷酸鈉，0.25M NaCl，pH 7.6

防腐劑： 無(wú)

Host: Human

Product: IgM (myeloma), whole molecule

Conjugate: Unconjugated

Product Category: ChromPure™ Purified Proteins from Normal Serums

RRID: AB_2337048

Information

ChromPure is our trade name for highly purified proteins from the serum of non-immunized animals.

Usage

Physical State: Sterile-filtered liquid

Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use.

Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.

Physical State: Sterile-filtered liquid

Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use.

Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.

Purity: Based on immunoelectrophoresis at an antigen concentration of 20 mg/ml, the pattern of precipitation against goat anti-human whole serum is the same as that against goat anti-human IgM, Fc5μ fragment specific. No precipitin line was detected against goat anti-human IgG, Fc fragment specific; goat anti-human IgA, a chain specific; or goat anti-humanalpha-2 macroglobulin.

Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6

Preservative: None

來(lái)源：https://www.amyjet.com/products/009-000-012.shtml