1.購買我公司任意ELISA試劑盒指標(biāo)享受免費代測，并提供技術(shù)指導(dǎo)服務(wù)！

二、實驗材料與試劑配制：

1. 儀器與材料：酶標(biāo)儀（使用前預(yù)熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時備用（如果標(biāo)本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人狼瘡抗凝抗體(LAC/LA)ELISA試劑盒廠家】三. 仔細(xì)閱讀說明書

1.檢查試劑盒標(biāo)簽上的有效期。如果超過有效期，請勿使用。

2. 按說明書確定所有試劑齊全（數(shù)量、體積）

3. 標(biāo)本制備要規(guī)范，每份標(biāo)本體積要按2-3個復(fù)孔以上的量制備（貯存），盡量分裝做備份。

4.短期內(nèi)無法實驗者，注意低溫保存

5.準(zhǔn)備好所有實驗額外所需物品，（比如移液器，試管，清洗器，酶標(biāo)儀）

6. 按說明書將所用試劑平衡至室溫，根據(jù)檢測標(biāo)本數(shù)量確定所需試劑的量

7.檢查試劑盒內(nèi)每種試劑的貯存條件，保證所有試劑均按照試劑盒內(nèi)說明書推薦的條件存儲。 【人狼瘡抗凝抗體(LAC/LA)ELISA試劑盒廠家】

8.檢查不穩(wěn)定或者變質(zhì)的試劑溶液（比如沉淀或變色）。有些試劑，比如標(biāo)準(zhǔn)品稀釋液或者檢測稀釋液，可能在設(shè)計時就含有沉淀物。所有試劑在滴入酶標(biāo)板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標(biāo)板之前，必需不停攪拌。

9.保證充分的孵育時間和溫度。

10.切勿使用不同生產(chǎn)批號的試劑取代現(xiàn)有試劑或混合現(xiàn)有試劑。

11.在混合或溶解蛋白溶液時，避免泡沫產(chǎn)生。

12.在實驗開始之前，安排好實驗流程。在實驗開始之前，清潔工作臺。

13.若有問題，應(yīng)與我公司或代理商的技術(shù)支持聯(lián)系

【人狼瘡抗凝抗體(LAC/LA)ELISA試劑盒廠家】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標(biāo)本前都必須有一個完整的計劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測，對收集后當(dāng)天就進(jìn)行檢測的標(biāo)本，及時儲存在4℃?zhèn)溆茫缬刑厥庠蛐枰芷谑占瘶?biāo)本，請造模取材后，將標(biāo)本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實驗質(zhì)量，所以避免反復(fù)凍融。部分標(biāo)本也可加入適當(dāng)防腐劑，激素類標(biāo)本需添加抑肽酶。 

2.試劑盒代測標(biāo)本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項請與我司技術(shù)人員溝通。

3.液體類標(biāo)本：標(biāo)本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。

4.購買前請?zhí)崆案嬖V我們：您樣本的種屬、樣本數(shù)量、待測指標(biāo)及其它特殊要求。

【人狼瘡抗凝抗體(LAC/LA)ELISA試劑盒廠家】五、代測數(shù)據(jù)詳細(xì)內(nèi)容

1、ELISA標(biāo)準(zhǔn)曲線；

2、實驗具體詳細(xì)步驟；

3、完整的實驗報告（試劑耗材，實驗方法，實驗結(jié)果及結(jié)果說明）；

4、實驗進(jìn)行階段，我公司客服人員會及時向您匯報實驗進(jìn)程。

9238854  #BR M96, Basic Agreement    The Basic Cover Agreement M96 provides full service coverage for the BioRobot M96. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238842  RS232 box, non-online fill, RapidPlate               CNY                             

9241215  Full Agreement Depot Repair, Shaker, 1yr       "The Full Agreement Depot Repair, Shaker provides full service coverage for the plate shaker used in the digene HPV application. The service is conducted as an instrument exchange service.

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."          CNY                             

9238833  #BR 9604, Basic Agreement    The Basic Cover Agreement 9604, 1 PM provides full service coverage for the BioRobot 9604. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9238832  BR 9600, Basic Agreement      The Basic Cover Agreement 9600 provides full service coverage for the BioRobot 9600. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                             【人狼瘡抗凝抗體(LAC/LA)ELISA試劑盒廠家】

9238819  Rect. carrier for 5 glass tubes 25ml                   CNY                             

9237527  Software LiquiChip Workstation IS V2.2.          CNY                             

9237380  Installation, BR MDx & 2 react. setup     Installation ａnd training, including two customized reaction setup protocols         CNY                             

9237379  Installation, BR MDx & 1 react. setup     Installation ａnd training, including one customized reaction setup protocol CNY                             

9237378  BR MDx, Installation        The Installation, BR MDx facilitates the quick implementation of the BioRobot MDx into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the BioRobot MDx instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.         CNY                             

9237175  Guard Fan, Platic XYP                CNY                             

9233209  Tubing, 3/2,                CNY                             

9233155  Power Cord, European              CNY                             

9236418  Board, CPU, TWI               CNY                             

9236345  Red LSR             CNY                             

9236166  Safety shield, RapidPlate          CNY                             

9230863  Anpressplatte Rose-80              CNY                             

9235538  Teaching Tool            CNY                             

9230282  Cable, RS323, robot to syringe pump, 225                CNY                             

5050-2002        hc2 Software V2 Package                  CNY                             

BS96RNA BioSprint 96 to RNA (384) - UDP               CNY                              【人狼瘡抗凝抗體(LAC/LA)ELISA試劑盒廠家】

9240730  Tubing, 1/4" OD, Red, AP          CNY                             

9240713  Poly Muffler Kit, AP96/196                 CNY                             

9240690  Bearings, Lead Screws, AP                CNY                             

9243654  Comprehen Valid., Other Quant. Sys.      """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   For planning purposes ａnd to ensure a smooth process, the Customer is expected to have all materials on-hand ａnd inventoried one (1) week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """     CNY                             

6000-5013        LumiCheck Plate User Package                  CNY                             

288103     bactotype Mycoplasma Mg/Ms PCR Kit (24)            CNY                             

1048147  QIAamp Viral RNA Mini Accessory Set              CNY                             

270043     cattletype BHV1 gB Ab (5)                CNY                             

1019992  Buffer Set (RNeasy 96 BioRobot 9604 Kit                  CNY                             

273401     pigtype Toxoplasma Ab (1)                CNY                             

QY-x1888 MCF-7/Adr 乳腺腺癌/阿霉素耐藥株                                                        

QY-x1889 KB 口腔表皮樣癌 SK-BR-3 乳腺腺癌                                                       

QY-x1890 Hep-2 喉癌 A2780 卵巢癌                                                 【人狼瘡抗凝抗體(LAC/LA)ELISA試劑盒廠家】

QY-x1891 Ec109 食管癌 CoC1卵巢癌                                               

QY-x1892 BGC-823 胃腺癌 CoC2卵巢癌                                                    

QY-x1893 MGC-803 胃腺癌 OVCAR-3 卵巢癌                                                 

QY-x1894 SGC7901 胃低分化腺癌 SK-OV-3 卵巢腺癌                                                      

QY-x1895 HuTu-80 十二指腸腺癌 SKOV-3/DDP 卵巢癌順鉑耐藥株                                                       

QY-x1896 Caco-2 結(jié)腸癌 HeLa 宮頸癌                                                    

QY-x1897 COLO 205 結(jié)腸癌 HelaS3 宮頸癌                                                     

QY-x1898 COLO 320DM 結(jié)腸癌 HEC-1B 子宮內(nèi)膜癌                                                      

QY-x1899 HCT-8 回盲腸腺癌 JEG-3 絨癌                                                 

QY-x1900 HCT-116 低分化結(jié)腸腺癌 Du-145前列腺癌                                                   

QY-x1901 HT-29 結(jié)腸腺癌 LNCaP 前列腺癌                                                    

QY-x1902 LoVo 結(jié)腸癌 LNCaP clone FGC 前列腺癌                                                

QY-x1903 SW480 結(jié)腸癌                                                      

QY-x1904 PC-3 前列腺癌                                                  

QY-x1905 BEL-7402 肝細(xì)胞癌 Tsu-Prl非雄激素依賴型前列腺癌                                                   

QY-x1906 HCC-9204 肝癌HOS 骨肉瘤                                                       【人狼瘡抗凝抗體(LAC/LA)ELISA試劑盒廠家】

QY-x1907 Hep3B 肝癌 MG-63 成骨肉瘤                                                 

QY-x1908 Hep G2 肝癌 Saos-2                                                    

QY-x1909 骨肉瘤                                                        

QY-x1910 SMMC-7721 肝癌 U-2 OS 骨肉瘤                                                     

QY-x1911 PANC-1胰腺導(dǎo)管上皮癌OS-732 骨肉瘤（瘤株）                                                  

QY-x1912 Panc 10.05 胰腺腺癌 A-204 橫紋肌肉瘤                                                

QY-x1913 SW1990胰腺腺癌HT1080纖維肉瘤                                                  

QY-x1914 SK-RC-42 腎癌 A375 皮膚黑色素瘤                                                

QY-x1915 RCC-krause 腎癌 A875 黑色素瘤                                                     

QY-x1916 Ketr-3 腎癌（瘤株） SK-HEL-1 皮膚黑色素瘤                                                        

QY-x1917 SW 13 腎上腺皮質(zhì)瘤 A431 皮膚基底細(xì)胞癌                                                 

QY-x1918 95-C 低轉(zhuǎn)移肺癌 CEM 白血病                                                

QY-x1919 95-D 高轉(zhuǎn)移肺癌 K562 慢性髓原白血病                                                

QY-x1920 973 肺腺癌 HPB-ALLT細(xì)胞白血病