價格詳細資料：試劑盒價格電議，齊一生物銷售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認同。齊一生物大品牌保證，傾力為國內(nèi)外科研院校實驗室提供*優(yōu)質(zhì)產(chǎn)品。

【人抑制素A(INH-A)ELISA試劑盒廠家】注意事項

1. 當(dāng)混合蛋白溶液時應(yīng)盡量輕緩，避免起泡。

2. 洗滌過程非常重要，不充分的洗滌易造成假陽性。

3. 一次加樣時間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。

4. 請每次測定的同時做標(biāo)準(zhǔn)曲線，做復(fù)孔。

5. 如標(biāo)本中待測物質(zhì)含量過高，請先稀釋后再測定，計算時請*后乘以稀釋倍數(shù)。

6. 在配制標(biāo)準(zhǔn)品、檢測溶液工作液時，請以相應(yīng)的稀釋液配制，不能混淆。

7. 底物請避光保存。

8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【人抑制素A(INH-A)ELISA試劑盒廠家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【人抑制素A(INH-A)ELISA試劑盒廠家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【人抑制素A(INH-A)ELISA試劑盒廠家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

QY112-1   高純度酵母質(zhì)粒大提試劑盒         10T                                       

QY113-1   革蘭氏陽性菌質(zhì)粒小量提取試劑盒     "100T

"                                            

QY114-1   高純度革蘭氏陽性菌質(zhì)粒小量提取試劑盒         "100T

"                                            

QY115-1   革蘭氏陽性菌質(zhì)粒中量提取試劑盒     "100T

"                                            

QY116-1   高純度革蘭氏陽性菌質(zhì)粒中量提取試劑盒         "100T

"                                            

QY117-1   革蘭氏陽性菌質(zhì)粒大量提取試劑盒     10T                                       

QY118-1   高純度革蘭氏陽性菌質(zhì)粒大量提取試劑盒         10T                                       

QY119-1   96普通質(zhì)粒小提試劑盒        "2板

"                                            

QY120-1   96酵母革蘭氏陽性菌質(zhì)粒小提試劑盒        "2板

"                                            

QY201-1   小量DNA產(chǎn)物純化試劑盒   "100T

"                                            

QY202-1   小量瓊脂糖凝膠DNA回收試劑盒        "100T

"                                            

QY203-1   大量DNA產(chǎn)物純化試劑盒   "100T

"                                            

QY204-1   多功能DNA純化回收試劑盒        "100T

"                                            

QY205-1   小分子量DNA，片段純化回收試劑盒         "100T

"                                             【人抑制素A(INH-A)ELISA試劑盒廠家】

QY206-1   玻璃奶DNA產(chǎn)物純化回收劑盒   "100T

"                                            

QY301-1   細菌基因組DNA提取試劑盒        "100T

"                                            

QY302-1   酵母基因組DNA提取試劑盒        "100T

"                                            

QY303-1   革蘭氏陽性細菌基因組DNA提取試劑盒   "100T

"                                            

QY304-1   血液基因組DNA小量提取試劑盒(離心柱型)     "100T

"                                            

QY305-1   血液基因組DNA中量提取試劑盒        "100T

"                                            

QY306-1   血液基因組DNA大量提取試劑盒(離心柱型)     "100T

"                                            

QY307-1   動物組織基因組提取試劑盒         "100T

"                                            

QY308-1   細胞基因組提取試劑盒         "100T

"                                            

QY309-1   植物基因組DNA提取試劑盒        "100T

"                                            

QY310-1   快捷植物基因組DNA提取試劑盒        "100T

"                                            

QY311-1   血液細胞組織基因組DNA提取試劑盒        "100T

"                                            

QY312-1   微量樣品基因組DNA提取試劑盒        "100T

"                                            

QY313-1   血液基因組DNA非離心柱型（0.1~20ml）         "100ML

"                                            

QY314-1   血凝塊基因組DNA提取試劑盒   "100T

"                                            

QY315-1   血漿游離基因組DNA提取試劑盒        "100T

"                                             【人抑制素A(INH-A)ELISA試劑盒廠家】

QY316-1   M13噬菌體單鏈基因組DNA快速提取試劑盒   "100T

"                                            

79523       Small Spin Columns  24 RNase-free spin columns for Oligotex spin procedures       CNY                             

76544       RNAprotect Animal Blood Tubes 100µl (50)              CNY                             

74324       RNeasy Protect Saliva Mini Kit (50) "The RNeasy Protect Saliva Kit is a D-BOM: RNAprotect Saliva Reagent (50ml) #1037475

RNeasy Micro kit (50)  #74004."   CNY                             

19590       InhibitEX (100)          CNY                             

70042       Oligotex mRNA Midi Kit  For 12 mRNA midipreps: 700 ul Oligotex Suspension, Small Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents ａnd Buffers   CNY                             

69984       TissueLyser Adapter Set (2 x 96)      2 sets of Adapter Plates for use with Collection Microtubes (racked) on the TissueLyser         CNY                             

69582       DNeasy 96 Blood & Tissue Kit (12)  For 12 x 96 DNA minipreps: 12 DNeasy 96 Plates, QIAGEN Proteinase K, Buffers, Square-Well Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Caps, 96-well Plate Registers       CNY                             

19581       96-Well Microplates (24) 96-well microplates with round-bottom wells plus lids, 24 per case, for use with QIAvac manifolds ａnd the BioRobot 9600    CNY                             

19418       QIAvac Holder Kit             CNY                             

19408       VacValves (24) For processing QIAamp mini spin columns on QIAvac 24 or QIAvace 24 Plus: 24 VacValves. Should be orded in conjunction with VacConnectors (500) - Catalog No. 19407.  CNY                              【人抑制素A(INH-A)ELISA試劑盒廠家】

19201       Collection Tubes (2-ml)     1000 Collection Tubes (2 ml)    CNY                             

19112       Buffer MTL (54 ml)            CNY                             

19046       Plasmid Buffer Set   Buffers P1, P2, P3, QBT, QC, QF, RNase A; for 100 plasmid mini-, 25 midi-, or 10 maxipreps CNY                             

16191       QIAwell 96 Ultra Plasmid Kit (4)       For 4 x 96 ultrapure plasmid minipreps, 4 each: QIAfilter 96, QIAwell 96, ａnd QIAprep 96 Plates; Reagents, Buffers, Collection Microtubes (1.2 ml), Caps     CNY                             

12991       QIAGEN Plasmid Plus Giga Kit (5)    QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega ａnd Giga) or up to 24 (Midi ａnd Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction.      CNY                             

12863       CompactPrep Plasmid Maxi Kit (25)         "For ultrafast purification of up to 200 µg or 750 µg Molecular Biology Grade plasmid or cosmid DNA for standard applications

CompactPrep Plasmid Kits enable fast, large-scale plasmid purification in a miniprep format. The use of a vacuum manifold allows 24 samples to be purified in parallel on a single workbench"       CNY                             

133281     NeXtalStock TRICINE pH 8.5 (200)            CNY                             

133275     NeXtalStock Spermidine (200)          CNY                             

337611     qBiomarker SYBR Green Mastermix (8)           CNY                             

4501303  artus EBV QS-RGQ Kit (24) RUO                CNY                             

4500363  artus HSV-1/2 QS-RGQ Kit (24) CE              CNY                             

949006     Autopure Cell Lysis Solution (3800 ml)               CNY                             

949002     Autopure Glycogen Solution (5 ml)           CNY                             

336313     SureSilencing shRNA Plasmid, Neomycin                   CNY                             

990382     Sample Tubes CB (2ml)              CNY                             

937255     QIAsymphony DSP DNA Midi Kit               CNY                              【人抑制素A(INH-A)ELISA試劑盒廠家】

9010815  Cable, card-X to bar-code camera BL180                  CNY                             

330619     RT² SYBR Green Fluor FAST Mastermix 25ml            CNY                             

133245     NeXtalStock Sodium malonate pH 7.0 (200)             CNY                             

929703     QX 0.2 ml 12-Tube Strip (80)             CNY                             

330512     RT² SYBR Green Fluor qPCR Mastermix (12)             CNY                             

330141     Custom RT² PreAMP Primer Mix               CNY                             

9010603  Anschlagleiste fuer Samtrak P7xxx (9194)                 CNY