1. 酶標儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【人抗副流感病毒IgG抗體(anti-PIVIgG)ELISA試劑盒現(xiàn)貨】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

ELISA應用：

ELISA檢測試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯(lián)免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認可及推崇,在歐美及中國獲得很大的推廣,尤其是國內(nèi)生化領域的長足發(fā)展.Elisa生物試驗是一種敏感性高,特異性強,重復性好的實驗診斷方法.由于其試劑穩(wěn)定、易保存,操作簡便,結果判斷較客觀等因素,已廣泛應用在免疫學檢驗的各領域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【人抗副流感病毒IgG抗體(anti-PIVIgG)ELISA試劑盒現(xiàn)貨】齊一生物科技歡迎您訂購咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應的ELISA試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【人抗副流感病毒IgG抗體(anti-PIVIgG)ELISA試劑盒現(xiàn)貨】齊一生物供應的ELISA試劑盒ELISA實驗原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實驗（ELISA）.已知濃度的標準品、未知濃度的樣品加入微孔酶標板內(nèi)進行檢測.先將生物素標記的抗體同時溫育.洗滌后,加入親和素標記過的HRP.再經(jīng)過溫育和洗滌,去除未結合的酶結合物,然后加入底物A、B,和酶結合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關系.

免疫測定技術的基礎在于抗原抗體之間的特異結合反應,所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術得到的單克隆抗體,而抗原或抗體的標記物如酶標結合物則通過各種化學合成方法制備.近年來,隨著分子生物學的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結合物等免疫測定試劑幾成為現(xiàn)實的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).

HBsAg試劑特點（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應性.酶表抗體為與HBsAg有不同結合位點的鼠復合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應用ParlEhrich學說（PEI）HBsAg標準品,對ad標準品的靈敏度為0.05ng/ml對ay標準品靈敏度為0.025ng/ml

ELISA檢測試劑盒應用定性夾心免疫檢測技術,用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標準品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結合物,經(jīng)第二次孵育后,酶結合物就會與次孵育結合上的HEVIgM抗體相結合,洗板除去未結合的酶結合物,加入TMB底物溶液,在第三次孵育時會發(fā)生酶-底物反應,只有那些含有HEVIgM抗體和酶結合物所形成的復合物的孔才會發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應,并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標準,O.D.值大于或等于Cut-Off值的樣品被認為是初試陽性. 【人抗副流感病毒IgG抗體(anti-PIVIgG)ELISA試劑盒現(xiàn)貨】

以上資料僅供參考了解，不供于論文寫作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長期經(jīng)營ELISA試劑盒（免費代測）及抗體、分子學生物試劑盒、金標法檢測試劑盒、比色法檢測試劑盒、細胞菌株、培養(yǎng)基、細胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

QY-x2217 B95-8 絨猴EBV轉化的淋巴細胞                                                       

QY-x2218 BABL/C1 BABL/C小鼠13-14天胚胎細胞                                                    

QY-x2219 BABL/C2 BABL/C小鼠胚胎細胞                                                   

QY-x2220 BABL/3T3 BABL/C小鼠胚胎成纖維細胞                                                      

QY-x2221 BALL-1 人B淋巴細胞急性白血病細胞                                                      

QY-x2222 Bcap-37 人乳腺癌細胞                                               

QY-x2223 BHK 敘利亞倉鼠腎細胞                                                      

QY-x2224 BHK-21 敘利亞倉鼠腎細胞                                                

QY-x2225 BIU-87 人淺表性膀胱癌細胞                                                      

QY-x2226 BRL-3A 大鼠肝細胞                                                      【人抗副流感病毒IgG抗體(anti-PIVIgG)ELISA試劑盒現(xiàn)貨】

QY-x2227 BT-325 人腦多形性膠質(zhì)母細胞瘤細胞                                                      

QY-x2228 BV-2 小鼠小腦膠質(zhì)瘤細胞                                                 

QY-x2229 BxPC3 人原位胰腺癌細胞                                                  

QY-x2230 C127 小鼠乳腺癌細胞                                                

QY-x2231 C6/36 白紋伊蚊細胞                                                    

QY-x2232 C6-121 大鼠神經(jīng)膠質(zhì)瘤細胞                                                      

QY-x2233 C6-1752 大鼠神經(jīng)膠質(zhì)瘤細胞                                                   

QY-x2234 C6-64 大鼠神經(jīng)膠質(zhì)瘤細胞                                               

QY-x2235 C6-70 大鼠神經(jīng)膠質(zhì)瘤細胞                                               

QY-x2236 C6-D2B 大鼠神經(jīng)膠質(zhì)瘤細胞                                                     

QY-x2237 C8166-CD4 人T細胞性白血病細胞                                                   

QY-x2238 CA 人肝癌細胞                                                    

QY-x2239 Caco-2 人結腸腺癌細胞                                                      

QY-x2240 CAM191 EBV-轉化人淋巴細胞                                                     

QY-x2241 CAM200 EBV-轉化人淋巴細胞                                                     

QY-x2242 CatK2 家貓腎上皮細胞                                               【人抗副流感病毒IgG抗體(anti-PIVIgG)ELISA試劑盒現(xiàn)貨】

QY-x2243 CatL1 家貓肺成纖維樣細胞                                               

QY-x2244 CatL2 家貓肺成纖維樣細胞                                               

QY-x2245 CatS2 家貓皮膚成纖維樣細胞                                                   

QY-x2246 CFPAC-1 人胰腺癌細胞                                               

QY-x2247 CHL 中國倉鼠肺細胞                                                  

9240576  Cable Assy, Capper, X51,CCW, AP96/196           CNY                             

9240546  Motor, Shutter Door, Eppendorf, AP196/98               CNY                             

9240544  Centrifuge, 5810RA, Eppendorf, AP196/98                CNY                             

9240513  Mouse, Standard, AP                 CNY                             

9240508  Software & Std. Protocols, AP98               CNY                             

9240499  Reagent Pump, AP            CNY                             

9240378  Autopure, Installation      The Installation, AUTOPURE facilitates the quick implementation of the AUTOPURE into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the AUTPOURE instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.         CNY                             

9240242  Connector, disp. tip adapter drive, TR              CNY                             

9239959  EZ1, Premium Agreement       The Premium Cover Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visits, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).         CNY                             

9020028  ZB sensor, 320mm, EZ1xl                   CNY                             

9020018  Upgrade kit, vacuum chamber, CAS-1820                 CNY                             

9019987  PCB, small breakout, 8 core, Cxx               CNY                             

9019927  Cover, tip ejector (UV), Cx8               CNY                             

9019926  PCB, Microboard, Cxx                CNY                              【人抗副流感病毒IgG抗體(anti-PIVIgG)ELISA試劑盒現(xiàn)貨】

9019488  Filter, polycap incl. tube/coupling, CX1             CNY                             

9238856  #Basic Cover Agreement MDx, 2PM, 1yr         The Basic Cover Agreement MDx, 2PM provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes two annual preventive maintenance visits, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9238839  #Twister II, Basic Agreement, no PM      The Basic Cover Agreement BR Twister II provides full service coverage for the BioRobot Twister II. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Cover Agreement, the response time is 5 working days.     CNY                             

9241178  Fuse, 4A, 250VAC, QX                CNY                             

9238830  BR 3000, Basic Agreement      The Basic Cover Agreement 3000 provides full service coverage for the BioRobot 3000. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238813  Rectangular carrier for 20 RIA-tubes                CNY                             

9019471  Lid, Polycarb, C1x              CNY                             

9237327  Upgrade, BR & 1 ext. device. w. sched.  Upgrade BioRobot with one approved integration device using scheduling software         CNY                             

9233197  Pump, vacuum, auto vac, BR8000            CNY                             

9237153  Syringe Pump            CNY                             

9237111  Upgrade App., QIAamp 96 Virus     On-site application Upgrade for standard QIAGEN QIAamp 96 DNA Virus  including kit         CNY                             

9237104  Upgrade, BR plus 4 ext. devices       Upgrade BioRobot with four approved integration devices    CNY                             

9237085  LiquiChip, Installation       The Installation, LiquiChip facilitates the quick implementation of the LiquiChip into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the LiquiChip Advanced instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.         CNY                             

9236420  Pins, rack alignment, set (5), TWI             CNY                             

9236412  Fingers, landscape, kit, TWI              CNY                             

9232975  Stirrer Adapter Board (Base Plate) PK80                   CNY                              【人抗副流感病毒IgG抗體(anti-PIVIgG)ELISA試劑盒現(xiàn)貨】

9232952  YZ 96 Connector Board 3 (Arm), E10085                   CNY                             

9236361  Alignment tube XYP                   CNY                             

9232938  Camera, buffer track, BR8000                   CNY