齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1。我必須運(yùn)行所有的標(biāo)準(zhǔn)和樣品的重復(fù)？

是的，重復(fù)的運(yùn)行，以監(jiān)測(cè)測(cè)定的精度和提高檢測(cè)結(jié)果的信心。

2。我必須在一次運(yùn)行所有的樣品嗎？

不，每個(gè)試劑盒采用stripwell微孔板。這允許用戶在不同的時(shí)間來分析不同數(shù)量的樣品。

3。什么類型的可重復(fù)性的結(jié)果，得到與分析？

每一個(gè)工具都有一個(gè)包含一個(gè)典型數(shù)據(jù)的圖表的手冊(cè)。在運(yùn)營商的任何變化，移液和洗滌技術(shù)，培養(yǎng)時(shí)間、溫度、試劑盒的年齡可以引起變化的結(jié)果。每個(gè)用戶都應(yīng)該得到自己的標(biāo)準(zhǔn)曲線。

4。是否有可能儲(chǔ)存的試劑比表示？

在不推薦使用條件下的部件組件的存儲(chǔ)，以保證測(cè)試的正確性能。

5。我應(yīng)該如何儲(chǔ)存我的樣品？

樣品應(yīng)保存在-20°C或更低的溫度。長期儲(chǔ)存，建議凍結(jié)他們?cè)?/span>- 70余。

6。我可以修改協(xié)議嗎？

BG的ELISA試劑盒進(jìn)行了優(yōu)化，提供*佳的結(jié)果。修改的格式或協(xié)議可能會(huì)給出錯(cuò)誤的結(jié)果。

7。我可以使用一個(gè)不建議在試劑盒插入的樣本類型嗎？

該試劑盒已被驗(yàn)證在試劑盒插入的樣品類型。未經(jīng)檢驗(yàn)的樣品類型。為進(jìn)一步信息的聯(lián)系技術(shù)服務(wù)。

8。我的樣本產(chǎn)生的值，在動(dòng)態(tài)范圍內(nèi)的測(cè)定。我可以使用這些值嗎？

建議只有在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降的樣本值。標(biāo)準(zhǔn)曲線的范圍內(nèi)的值一般是非線性的，這可能導(dǎo)致不正確的外推值。產(chǎn)生值高于標(biāo)準(zhǔn)的樣品應(yīng)為（進(jìn)一步）稀釋和重復(fù)。如果樣品低于該測(cè)定的范圍內(nèi)，該樣品被認(rèn)為是不可檢測(cè)的。

9。我每次都要運(yùn)行一個(gè)空白或零標(biāo)準(zhǔn)嗎？

是的，這些都是必需的計(jì)算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測(cè)到檢測(cè)。當(dāng)排除特定的檢測(cè)問題的來源時(shí)，它們也非常有幫助。

10。我能改變我在檢測(cè)中使用的樣本量嗎？

不建議你改變卷由于BG工具被設(shè)計(jì)為*佳的性能在給定的體積

11�？梢允褂貌煌慕M件組件嗎？

每一個(gè)組件包含有特定批號(hào)的組件，以確保所有的組件都進(jìn)行優(yōu)化，以及與組件中的所有其他組件。對(duì)這些特定的地段進(jìn)行質(zhì)量檢驗(yàn)。這是從來沒有建議使用你自己的組件或組件從其他包或供應(yīng)商。

12。我的標(biāo)準(zhǔn)曲線看起來很好，但我沒有得到一個(gè)信號(hào)，我的樣本，當(dāng)我預(yù)期，為什么？

樣品不包含被分析物。一個(gè)矩陣的效果可能是掩蓋檢測(cè)。確保推薦的稀釋液在試劑盒插入后按規(guī)定進(jìn)行。如果建議稀釋，請(qǐng)檢查是否正確進(jìn)行稀釋。過稀釋會(huì)導(dǎo)致樣品在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降。

13。你怎么推薦我洗盤子？

如果您使用的是一個(gè)自動(dòng)平板洗衣機(jī)，我們建議定期檢查校準(zhǔn)，并將該系統(tǒng)在洗滌前沖洗緩沖液沖洗。手動(dòng)洗衣機(jī)也是一樣的。一個(gè)中繼器或洗瓶也可以使用。用戶應(yīng)小心，以確保所有的內(nèi)容都是送氣和板拍干的無塵紙。【人抗SS-B/La抗體ELISA試劑盒現(xiàn)貨】

14。我需要用一個(gè)盤子嗎？

可靠的結(jié)果可以得到無板振動(dòng)，但外徑的一般會(huì)低于那些使用平板振動(dòng)器。

15。為什么我要用波長校正450-570nm之間？

對(duì)于酶聯(lián)免疫吸附試驗(yàn)，在雙波長讀的是做正確的光密度貢獻(xiàn)的塑料以及，燈和光學(xué)波動(dòng)。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協(xié)議使用。稀釋或濃縮的金額將由*終用戶決定。

17。什么是預(yù)期的分析物濃度，我應(yīng)該找到？

一個(gè)給定的分析物的量可能會(huì)有所不同，不僅從物種的物種，但也組織和細(xì)胞來源。這個(gè)信息的*佳來源是目前的文獻(xiàn)，很容易通過互聯(lián)網(wǎng)在多個(gè)科學(xué)數(shù)據(jù)庫訪問。

18。我的光密度有點(diǎn)高（或較低），比那些在手冊(cè)中，與我的試劑盒來了。為什么？

光學(xué)密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【人抗SS-B/La抗體ELISA試劑盒現(xiàn)貨】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【人抗SS-B/La抗體ELISA試劑盒現(xiàn)貨】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【人抗SS-B/La抗體ELISA試劑盒現(xiàn)貨】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

QY-x1076 人肺腺癌細(xì)胞（胸膜滲出液）Calu-3                                                

QY-x1077 人肺癌細(xì)胞系（未分化）Calu-6                                                

QY-x1078 人胚肺二倍體細(xì)胞HLF-02                                                  

QY-x1079 人肺癌細(xì)胞系Tul-1                                                       【人抗SS-B/La抗體ELISA試劑盒現(xiàn)貨】

QY-x1080 人胚肺成纖維細(xì)胞HFL-I                                                      

QY-x1081 人胚肺細(xì)胞MRC-5                                                       

2QY-x1082        人胚肺細(xì)胞WI-38                                                       

QY-x1083 人SV－40轉(zhuǎn)化肺成纖維細(xì)胞 WI－38AV13                                                      

QY-x1084 人肺癌亞歷山大細(xì)胞系PLC/PRF/5                                                      

QY-x1085 人肺巨細(xì)胞癌(PG)的低轉(zhuǎn)移亞系PG-LH7                                                   

QY-x1086 人肺巨細(xì)胞癌(PG）的高轉(zhuǎn)移亞系PG-BE1                                               

QY-x1087 人肺腺癌細(xì)胞系PG49                                                 

QY-x1088 人肺腺癌細(xì)胞GLC-82                                                  

QY-x1089 人肺巨細(xì)胞癌細(xì)胞HLAmP                                                  

QY-x1090 人肺腺癌細(xì)胞（轉(zhuǎn)染TK基因的GLC-82細(xì)胞）GLC-82-TK                                              

QY-x1091 人胚肺成纖維細(xì)胞突變癌細(xì)胞Z-HL16C                                                    

QY-x1092 人肺動(dòng)脈內(nèi)皮細(xì)胞HPAEC                                                   

QY-x1093 人肺泡上皮細(xì)胞HPAEpiC                                                    

QY-x1094 人胚肺成纖維細(xì)胞CCC-HPF-1                                                     

QY-x1095 人低分化肺腺癌細(xì)胞SK-LU-1                                                       【人抗SS-B/La抗體ELISA試劑盒現(xiàn)貨】

QY-x1096 人肺鱗癌細(xì)胞LTEP-s                                                    

QY-x1097 人小細(xì)胞肺癌細(xì)胞LTEP-sm                                                

QY-x1098 人小細(xì)胞肺癌細(xì)胞LTEP-P                                                   

QY-x1099 人肺癌細(xì)胞973                                                    

QY-x1100 人胚肺成纖維細(xì)胞　HELF                                                  

QY-x1101 人低轉(zhuǎn)移肺癌細(xì)胞95-C                                                       

QY-x1102 人高轉(zhuǎn)移肺癌細(xì)胞95-D                                                      

QY-x1103 人肺腺癌細(xì)胞A2                                                 

QY-x1104 人支氣管上皮細(xì)胞HBE                                                       

QY-x1105 人支氣管上皮細(xì)胞16HBE                                                   

QY-x1106 人腎癌細(xì)胞Ketr-3                                               

9234860  Tubing coupling piece for system liquid             CNY                             

9238666  Pneumatic spring, 200 Nm                CNY                             

9238661  Filter dryer                CNY                             

9238660  Temperature sensor                  CNY                              【人抗SS-B/La抗體ELISA試劑盒現(xiàn)貨】

9242029  QIAxcel, Repair Fee "Repair service delivery send to Regional Repair Center

Service response time (repair) 7-10 days turnaround time

Periodic inspection/maintenance: 0

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9241895  QIArent&go - Rent out fee QIAsymphony AS           CNY                             

9241814  Pyro Q24, PM Agreement        An agreement for preventive maintenance provides coverage for travel, labor, ａnd parts, plus the cost of consumables required for the maintenance visit. Work carried out during the preventive maintenance visit is fully documented in accordance with GMP/GLP directives.   CNY                             

9241165  Board, smart key, QX                 CNY                             

9019855  72-well High Profile Locking Ring              CNY                             

9019833  Cable, RS232,  external, V2, QX              CNY                             

9019763  Motor, Y, V2, QX                CNY                             

9019732  Board, LED scan, V2, QX           CNY                             

9013659  BGR DT Adapter 1100ul P3xxx                  CNY                             

9013444  Reagent holder, 3-trough 20 ml                CNY                             

9013437  Maintenance kit,  annual, BR9600 (F)            CNY                             

9013426  Maintenance Kit BR 9600 (C)           CNY                             

9019661  Manometer, PyroQ96VW                  CNY                             

9019659  Screw set VPT, PyroQ96VW              CNY                             

9019654  Springloaded pins VPT, PyroQ96VW                  CNY                             

9019620  Belt, Z-axis assembly, 1 ch., Cx2                CNY                              【人抗SS-B/La抗體ELISA試劑盒現(xiàn)貨】

9019606  Pipettor, Piston, Cx2                  CNY                             

9019576  Opto switch, Y-axis, Cxx            CNY                             

9019527  PLATE SEPARATOR 96 WELL WHITE                   CNY                             

9019525  UV support frame, QIA             CNY                             

9019519  ZLV ADAPTER KIT HIGH SKIRT                   CNY                             

204143     QuantiTect SYBR Green PCR Kit (200)     For 200 x 50 ul reactions: 3 x 1.7 ml QuantiTect SYBR Green PCR Master Mix,* 2 x 2.0 ml RNase-free water CNY                             

9019438  Fan 115VAC   50/60 Hz  0.26A              CNY                             

9019409  Sensor, rotor timing & home, RG3           CNY                             

9019407  Switch, mains voltage selector, RG3                 CNY                             

9019400  Sensor, infrared, RG3                CNY                             

9019361  Fan, Base, 12V 120mm, RG6            CNY                             

9019354  Coupling, rotor shaft, RG6                 CNY                             

1027299  AllStars Cell Death Contr.siRNA (20nmol)                  CNY