1.購(gòu)買(mǎi)我公司任意ELISA試劑盒指標(biāo)享受免費(fèi)代測(cè)，并提供技術(shù)指導(dǎo)服務(wù)！

二、實(shí)驗(yàn)材料與試劑配制：

1. 儀器與材料：酶標(biāo)儀（使用前預(yù)熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時(shí)備用（如果標(biāo)本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

【人細(xì)胞周期素D1(Cyclin-D1)ELISA試劑盒現(xiàn)貨】三. 仔細(xì)閱讀說(shuō)明書(shū)

1.檢查試劑盒標(biāo)簽上的有效期。如果超過(guò)有效期，請(qǐng)勿使用。

2. 按說(shuō)明書(shū)確定所有試劑齊全（數(shù)量、體積）

3. 標(biāo)本制備要規(guī)范，每份標(biāo)本體積要按2-3個(gè)復(fù)孔以上的量制備（貯存），盡量分裝做備份。

4.短期內(nèi)無(wú)法實(shí)驗(yàn)者，注意低溫保存

5.準(zhǔn)備好所有實(shí)驗(yàn)額外所需物品，（比如移液器，試管，清洗器，酶標(biāo)儀）

6. 按說(shuō)明書(shū)將所用試劑平衡至室溫，根據(jù)檢測(cè)標(biāo)本數(shù)量確定所需試劑的量

7.檢查試劑盒內(nèi)每種試劑的貯存條件，保證所有試劑均按照試劑盒內(nèi)說(shuō)明書(shū)推薦的條件存儲(chǔ)。 【人細(xì)胞周期素D1(Cyclin-D1)ELISA試劑盒現(xiàn)貨】

8.檢查不穩(wěn)定或者變質(zhì)的試劑溶液（比如沉淀或變色）。有些試劑，比如標(biāo)準(zhǔn)品稀釋液或者檢測(cè)稀釋液，可能在設(shè)計(jì)時(shí)就含有沉淀物。所有試劑在滴入酶標(biāo)板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標(biāo)板之前，必需不停攪拌。

9.保證充分的孵育時(shí)間和溫度。

10.切勿使用不同生產(chǎn)批號(hào)的試劑取代現(xiàn)有試劑或混合現(xiàn)有試劑。

11.在混合或溶解蛋白溶液時(shí)，避免泡沫產(chǎn)生。

12.在實(shí)驗(yàn)開(kāi)始之前，安排好實(shí)驗(yàn)流程。在實(shí)驗(yàn)開(kāi)始之前，清潔工作臺(tái)。

13.若有問(wèn)題，應(yīng)與我公司或代理商的技術(shù)支持聯(lián)系

【人細(xì)胞周期素D1(Cyclin-D1)ELISA試劑盒現(xiàn)貨】四、客戶(hù)須知

齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

1.在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃，必須清楚要檢測(cè)的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測(cè)，對(duì)收集后當(dāng)天就進(jìn)行檢測(cè)的標(biāo)本，及時(shí)儲(chǔ)存在4℃?zhèn)溆�，如有特殊原因需要周期收集�?biāo)本，請(qǐng)?jiān)炷Ｈ〔暮�，將�?biāo)本及時(shí)分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實(shí)驗(yàn)質(zhì)量，所以避免反復(fù)凍融。部分標(biāo)本也可加入適當(dāng)防腐劑，激素類(lèi)標(biāo)本需添加抑肽酶。 

2.試劑盒代測(cè)標(biāo)本的客戶(hù)取材前須向我司銷(xiāo)售人員索要說(shuō)明書(shū)，具體操作注意事項(xiàng)請(qǐng)與我司技術(shù)人員溝通。

3.液體類(lèi)標(biāo)本：標(biāo)本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。

4.購(gòu)買(mǎi)前請(qǐng)?zhí)崆案嬖V我們：您樣本的種屬、樣本數(shù)量、待測(cè)指標(biāo)及其它特殊要求。

【人細(xì)胞周期素D1(Cyclin-D1)ELISA試劑盒現(xiàn)貨】五、代測(cè)數(shù)據(jù)詳細(xì)內(nèi)容

1、ELISA標(biāo)準(zhǔn)曲線(xiàn)；

2、實(shí)驗(yàn)具體詳細(xì)步驟；

3、完整的實(shí)驗(yàn)報(bào)告（試劑耗材，實(shí)驗(yàn)方法，實(shí)驗(yàn)結(jié)果及結(jié)果說(shuō)明）；

4、實(shí)驗(yàn)進(jìn)行階段，我公司客服人員會(huì)及時(shí)向您匯報(bào)實(shí)驗(yàn)進(jìn)程。

HH   人肝細(xì)胞         Human Hepatocytes        2 x 10^6 cells/vial                                

HHSteC    人肝星形細(xì)胞         Human Hepatic Stellate Cells  5 x 10^5 cells/vial                                

HGBF        人膽囊成纖維細(xì)胞         Human Gallbladder Fibroblasts        5 x 10^5 cells per 1ml                                  

HSEC         人脾血管內(nèi)皮細(xì)胞         Human Splenic Endothelial Cells      5 x 10^5 cells/vial                                

HSF  人脾成纖維細(xì)胞     Human Splenic Fibroblasts       5 x 10^5 cells/vial                                

HCMEC     人心臟微血管內(nèi)皮細(xì)胞         Human Cardiac Microvascular Endothelial Cells      5 x 10^5 cells/vial                                

HCAEC      人冠狀動(dòng)脈內(nèi)皮細(xì)胞     Human Coronary Artery Endothelial Cells        5 x 10^5 cells/vial                                

HAEC        人主動(dòng)脈內(nèi)皮細(xì)胞         Human Aortic Endothelial Cells        5 x 10^5 cells/vial                                 【人細(xì)胞周期素D1(Cyclin-D1)ELISA試劑盒現(xiàn)貨】

HASMC     人主動(dòng)脈平滑肌細(xì)胞     Human Aortic Smooth Muscle Cells          5 x 10^5 cells/vial                                

HAF  人主動(dòng)脈成纖維細(xì)胞     Human Aortic Fibroblasts         5 x 10^5 cells/vial                                

HCM         人心肌細(xì)胞     Human Cardiac Myocytes        5 x 10^5 cells/vial                                

HCM-a      人心肌細(xì)胞－－成人  Human Cardiac Myocytes-adult       5 x 10^5 cells/vial                                

HCF  人心臟成纖維細(xì)胞         Human Cardiac Fibroblasts      5 x 10^5 cells/vial                                

HCF-av      人心臟成纖維細(xì)胞－－成人心室       Human Cardiac Fibroblasts-adult ventrical      5 x 10^5 cells/vial                                

HCF-aa     人心臟成纖維細(xì)胞－－成人心房       Human Cardiac Fibroblasts-adult atrial   5 x 10^5 cells/vial                                

HCF-a        人心臟成纖維細(xì)胞－－成人       Human Cardiac Fibroblasts-adult   5 x 10^5 cells/vial                                

HCF-fa      人心臟成纖維細(xì)胞－－胎兒心房       Human Cardiac Fibroblasts-fetal atrial         6 x 10^5 cells/vial                                

HPcF         人心包成纖維細(xì)胞         Human Pericardial Fibroblasts         5 x 10^5 cells/vial                                

HCEpiC     人角膜上皮細(xì)胞     Human Corneal Epithelial Cells         5 x 10^5 cells/vial                                

HK    人角膜細(xì)胞     Human Keratocytes          5 x 10^5 cells/vial                                

HREC        人視網(wǎng)膜內(nèi)皮細(xì)胞         Human Retinal Endothelial Cells      5 x 10^5 cells/vial                                

HRPEpiC   人視網(wǎng)膜色素上皮細(xì)胞         Human Retinal Pigment Epithelial Cells  5 x 10^5 cells/vial                                

HLEpiC      人晶狀體上皮細(xì)胞         Human Lens Epithelial Cells     5 x 10^5 cells/vial                                

HIPEpiC    人虹膜色素上皮細(xì)胞     Human Iris Pigment Epithelial Cells 5 x 10^5 cells/vial                                 【人細(xì)胞周期素D1(Cyclin-D1)ELISA試劑盒現(xiàn)貨】

HConF       人結(jié)膜成纖維細(xì)胞         Human Conjunctival Fibroblasts       5 x 10^5 cells/vial                                

HNPCEpiC         人無(wú)色素睫裝上皮細(xì)胞         Human Non-Pigment Ciliary Epithelial Cells     5 x 10^5 cells/vial                                

HTMC       人小梁網(wǎng)細(xì)胞         Human Trabecular Meshwork Cells 5 x 10^5 cells/vial                                

HOCF        人眼脈絡(luò)膜成纖維細(xì)胞         Human Ocular Choroid Fibroblasts  5 x 10^5 cells/vial                                

HConEpiC 人結(jié)膜上皮細(xì)胞     Human Conjunctival Epithelial Cells          5 x 10^5 cells/vial                                

HVCEC      人絨毛膜毛細(xì)血管內(nèi)皮細(xì)胞         Human Villus Capillary Endothelial Cells  5 x 10^5 cells/vial                                

HAmEpiC 人羊膜上皮細(xì)胞     Human Amniotic Epithelial Cells      5 x 10^5 cells/vial                                

HVT 人絨毛膜滋養(yǎng)層細(xì)胞     Human Villous Trophoblasts    1 x 10^6 cells/vial                                

HVMF       人絨毛間充質(zhì)成纖維細(xì)胞     Human Villous Mesenchymal Fibroblasts         5 x 10^5 cells/vial                                

9238610  Collet chuck               CNY                             

9238479  Motor, syringe, RapidPlate               CNY                             

9238152  Elution Microplate Adapter 8000, SBS              CNY                             

9237932  Syringe, 100µl           CNY                             

9240663  Nut, Flangeless,1/4-28, Black, AP              CNY                             

9240634  Power Supply, Main PCB, Aztec, AP196/98                CNY                             

9240630  Power Supply, 5VDC 1.5 AMP, AP96                  CNY                             

9240593  Unload Valve, Pump, Pressure, AP196/98                  CNY                             

9240562  Fuse, PCB, 315, Eppendorf, AP196/98                CNY                             

9240536  Motor Assy, SiNug, 17, All, AP96/196                 CNY                             

9240514  CD R/W, AP196/98             CNY                             

9240504  PC-104, AP98            CNY                             

9240346  Autopure, Full Agreement       "Repair service delivery: onsite

Service response time (repair): 48 hours/2 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"        CNY                             

9240098  Re-Installation, BR EZ1    The Re-Installation, BR EZ1 facilitates quick implementation of system back into lab routine by minimizing  instrument set-up phase. It covers hardware ａnd software check of instrument ａnd provides user with operator refesher overview.  Re-installation includes labor ａnd travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.          CNY                             

9239852  Foot bumpers,  v2.0 (4)          CNY                              【人細(xì)胞周期素D1(Cyclin-D1)ELISA試劑盒現(xiàn)貨】

9019944  Cable, P-axis, wires, 8 ch. , C18                 CNY                             

9238880  Bearings up. & low, BS96                   CNY                             

9238836  BioSprint96, Basic Agreement, no PM    "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: not applicable"        CNY                             

9241211  Full Agreement Depot Repair, DML2000, 1y  "The Full Agreement Depot Repair, DML2000 provides full service coverage for the DML 2000 luminometer. The service is conducted as an instrument exchange service.

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."          CNY                             

9241191  Magnet, tool, QX              CNY                             

9238825  Spare bucket for plate rotor             CNY                             

9238815  Rect. carrier for 12 tubes 10 ml w/ cap            CNY                             

9019472  TAPE SPACER CLEAR GS             CNY                             

9019464  UV tube, Cxx             CNY                             

9243650  Comprehen Valid., EZ1/QIAcube/QIAsymph    """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   The Customer is expected to have all materials on-hand 1 week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """     CNY                             

9237333  Install, BR plus 3 integr. w. scheduling     Installation ａnd training, BioRobot System including three approved integration devices with scheduling   CNY                             

9237328  Upgrade, BR & 2 ext. devices w. sched.  Upgrade BioRobot with two approved integration devices using scheduling software  CNY                             

9237173  Shield, V-housing               CNY                             

9233208  Tubing, liquid system 2.5/1.5             CNY                             

9237155  V-Housing with Sample Needle                  CNY                             

9237150  Heater Top                CNY                              【人細(xì)胞周期素D1(Cyclin-D1)ELISA試劑盒現(xiàn)貨】