ELISA酶聯(lián)法試劑盒操作因固相載體的形成不同而有所差異，國內(nèi)醫(yī)學(xué)檢驗一般均用板式點。本文將敘述板式ELISA各個操作步驟的注意要點，珠式、管式及磁性球ELSIA，國外試劑均與特殊儀器配合應(yīng)用，兩者均有詳細(xì)的使用說明，嚴(yán)格遵照規(guī)定操作，必能得出準(zhǔn)確的結(jié)果。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

首先齊一生物優(yōu)質(zhì)的試劑盒，良好的儀器和正確的操作是保證ELISA檢測結(jié)果準(zhǔn)確可靠必要條件。所以ELISA試劑盒的購買，試劑質(zhì)量的保證以及實驗前實驗儀器的檢察都是很有重要性的。保證實驗材料的質(zhì)量之后要嚴(yán)格按照實驗步驟進(jìn)行。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【大鼠粒細(xì)胞集落刺激因子(G-CSF)ELISA試劑盒現(xiàn)貨】齊一生物ELISA試劑盒

1.精心挑選的進(jìn)口抗體、標(biāo)準(zhǔn)品、顯色劑和酶標(biāo)板全部為進(jìn)口產(chǎn)品實現(xiàn)*佳性能；

2.原材料批量進(jìn)口加上自配的特殊配方稀釋劑保證高品質(zhì)產(chǎn)品性能的同時兼顧成本；

3.采用技術(shù)，嚴(yán)格按照標(biāo)準(zhǔn)生產(chǎn)方案組裝生產(chǎn)；

4.預(yù)包被微孔板的批內(nèi)和批間變異系數(shù)均小于10 ％；

5.經(jīng)過嚴(yán)格交叉反應(yīng)和干擾測試及穩(wěn)定性試驗；

6.明確的敏感度；

7.針對所有樣品類型達(dá)到*佳的性能；

8.廣泛的檢測范圍可以滿足大多數(shù)檢測要求；

9.完善的售后服務(wù)和免費的技術(shù)支持免去您的后顧之憂；

10.強(qiáng)大的生產(chǎn)能力和充足的儲備、操作熟練的技術(shù)人員及高效的生產(chǎn)流程，隨時可以組裝出您定制的產(chǎn)品。

【大鼠粒細(xì)胞集落刺激因子(G-CSF)ELISA試劑盒現(xiàn)貨】齊一生物僅供實驗人、大鼠、小鼠、兔等動物實驗標(biāo)本檢測使用！得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細(xì)閱讀購買說明！齊一生物銷售：021－6034 8496；181214 53965；173021 04490

ELISA試劑盒問題描述

標(biāo)準(zhǔn)曲線梯度差：

吸液或加液不準(zhǔn)請檢查移液器及吸頭

標(biāo)準(zhǔn)品稀釋不正確溶解標(biāo)準(zhǔn)品稍微旋轉(zhuǎn)瓶身，輕輕混勻使粉末完全溶解

洗滌不完全保證洗滌時間和洗滌次數(shù)及每孔的加液量

顯色很弱或無色：

孵育時間太短保證充足的孵育時間

實驗溫度不正確使用推薦的實驗溫度

試劑體積不夠或漏加、稀釋不正確檢查吸液及加液過程，保證所有試劑按順序足量添加

讀數(shù)數(shù)值低：

酶標(biāo)儀設(shè)置不正確 在酶標(biāo)儀上檢查波長及濾光片設(shè)置

提前打開酶標(biāo)儀預(yù)熱：變異系數(shù)大加液不正確檢查加液情況

【大鼠粒細(xì)胞集落刺激因子(G-CSF)ELISA試劑盒現(xiàn)貨】背景值高：

檢測抗體的工作濃度使用推薦的稀釋倍數(shù)

酶標(biāo)板洗滌不完全重新閱讀操作手冊，保證清洗完全；如果用自動洗板機(jī)，請檢查所有的出口是否有堵塞

洗液有污染配制新鮮的洗液

靈敏度低：

ELISA試劑盒保存按說明書要求保存相關(guān)試劑

讀數(shù)前未終止OD讀數(shù)前應(yīng)在每孔中加入終止液

齊一生物客戶寄標(biāo)本時需注明以下情況：

1、標(biāo)本編號；2、所測項目；3、是否做復(fù)孔；3、聯(lián)系方式；4、實驗后標(biāo)本是否寄回。

客戶必知：

客戶應(yīng)對所提供的材料及信息負(fù)責(zé)，如因客戶提供的材料及信息不準(zhǔn)確而引起的實驗延誤或經(jīng)濟(jì)損失由客戶承擔(dān)。

試劑盒樣本要求

在收集標(biāo)本前都必須有一個完整的計劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測，對收集后當(dāng)天就進(jìn)行檢測的標(biāo)本，及時儲存在4℃?zhèn)溆茫缬刑厥庠蛐枰芷谑占瘶?biāo)本，請造模取材后，將標(biāo)本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實驗質(zhì)量，所以避免反復(fù)凍融。具體操作注意事項請與我司技術(shù)人員溝通。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【大鼠粒細(xì)胞集落刺激因子(G-CSF)ELISA試劑盒現(xiàn)貨】液體類標(biāo)本：標(biāo)本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。

1. 血清：室溫血液自然凝固10-20分鐘后，離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成，應(yīng)再次離心。

2. 血漿：應(yīng)根據(jù)標(biāo)本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑，混合10-20分鐘后，離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成應(yīng)再次離心。

3. 尿液、胸腹水、腦脊液：用無菌管收集。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。如有沉淀形成，應(yīng)再次離心。

4. 細(xì)胞培養(yǎng)上清：檢測分泌性的成份時，用無菌管收集。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。

5. 培養(yǎng)細(xì)胞:檢測細(xì)胞內(nèi)的成份時，用PBS（PH7.2-7.4）稀釋細(xì)胞懸液，細(xì)胞濃度達(dá)到100萬/ml左右。通過反復(fù)凍融或加入組織蛋白萃取試劑，以使細(xì)胞破壞并放出細(xì)胞內(nèi)成份。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成，應(yīng)再次離心。

6. 組織標(biāo)本：切割標(biāo)本后，稱取重量。加入一定量的PBS，緩沖液中可加入1μg/L蛋白酶抑制劑或50U/ml的Aprotinin（抑肽酶）。用手工或勻漿器將標(biāo)本勻漿充分。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清置于-20度或-70度保存，如有必要，可以將樣品濃縮干燥。分裝后一份待檢測，其余冷凍備用。

7. 標(biāo)本采集后盡早進(jìn)行提取，提取按相關(guān)文獻(xiàn)進(jìn)行，提取后應(yīng)盡快進(jìn)行實驗。若不能馬上進(jìn)行試驗，可將標(biāo)本放于-20℃保存，但應(yīng)避免反復(fù)凍融

8.不能檢測含NaN3的樣品，因NaN3抑制辣根過氧化物酶的（HRP）活性。

9.如果樣品不能立即檢測，應(yīng)將其分裝，-70 ℃保存，避免反復(fù)冷凍。保存過程中如出現(xiàn)沉淀，應(yīng)再次離心。血液標(biāo)本盡可能的不要使用溶血或高血脂血。如果血清中含大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解

凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

9020596  Tape Foam,045X750 Double Stick,DML 3000            CNY                           

9017843  Tool, cable, communication, RS232, QIAC         CNY                           

9013401  Cable set, cooling system, 90/120        CNY                           

9234873  ZLVPipetting Station for 2 microtit.             CNY                           

9013395  Tip disposal bags           CNY                           

9013391  Tubing set, PVC, BR9604            CNY                           

9013268  Cable, card-X to solenoid valve, 24V           CNY                           

9013222  Holder, tube, STS, single, 11 ml (30), 0              CNY                            【大鼠粒細(xì)胞集落刺激因子(G-CSF)ELISA試劑盒現(xiàn)貨】

9013204  Transportation kit, BR8000           CNY                           

9011759  Reagent holder, 4-tube 13ml  Holder for accommodating 4 microcentrifuge tubes (13 ml) on the worktable; must be used with the reagent holder tray   CNY                           

9011672  Reader Abdeckung spez. GSG (9194)          CNY                           

132605    NeXtal Stock Sodium ac. H2O (50)      1.6M Sodium acetate trihydrate - 50mL (4 x 12,5 ml tube)  CNY                           

800141    Investigation grade sequencing, w/ prep       "Single-stranded sequencing of an unknown sequence

QIAGEN DNA template preparation

Design ａnd synthesis of walking primers

Data assembly ａnd sequence alignment

Accuracy >99%"    CNY                           

767134    PAXgene Tissue DNA Kit (50)             CNY                           

929605    QX Mineral Oil (50 ml)         CNY                           

330513    RT² SYBR Green Fluor qPCR Mastermix (24)            CNY                           

330502    RT² SYBR Green qPCR Mastermix (12)              CNY                           

330401    RT² First Strand Kit (12)              CNY                           

9010813  BGR Stirrer PK80          CNY                           

69506     DNeasy Blood & Tissue Kit (250) 250 DNeasy Spin Columns, QIAGEN Proteinase K, Buffers, Collection Tubes (2 ml)       CNY                           

63204     DyeEx 2.0 Spin Kit (50) 50 DyeEx Spin Columns, Collection Tubes (2 ml)       CNY                           

211392    Internal Control DNA (High conc.)              CNY                           

9012755  Set, Tubing, Washer, P3        CNY                           

9010126  Washer boat           CNY                            【大鼠粒細(xì)胞集落刺激因子(G-CSF)ELISA試劑盒現(xiàn)貨】

979015    MOTT 16S Primer 1 for Sequencing           CNY                           

979010    PyroMark Vacuum Prep Filter Probe (100)   Re-usable filter probes for PyroMark Vacuum Workstation Q96 ａnd Q24.       CNY                           

59720     EpiTect Fast 96 DNA Bisulfite Kit         CNY                           

9002245  QIAcube(220-240v) Promo, PrioPLUS QIAcube (220-240V) Promo Priority includes the QIAcube instrument +promo iPad, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 3 years. One preventive maintenance visit is included for each year of the warranty; 3 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.   CNY                           

9002198  RGQ 5plex HRM, PC, PrioPLUS, no PM     "RGQ 5plex, HRM, PC Instrument

Installation of RGQ 5plex HRM & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

Loaner available in 2 days*

No preventive Maintenance

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"     CNY                           

9002197  RGQ 5plex, PC, PrioPLUS, basic, no PM    "RGQ 5plex, PC Instrument

Installation of RGQ 2plex & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

No Loaner available

No Preventive Maintenance

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"     CNY                           

9012718  Pipetting Position (raised platform)              CNY                           

9002042  Rotor-Gene Q MDx 6plex Platform             CNY                           

9002038  Rotor-Gene Q MDx 5plex HRM, Priority     Rotor-Gene Q MDx 5plex HRM, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents. CNY                           

QY-18682       anti-Tie2（Porcine tyrosine kinase with immunoglobulin-like ａnd EGF-like domains 2）上皮生長因子樣域酪氨酸激酶2                                   

QY-18692       Anti-TIGAR humen (TP53- induced glycolysis ａnd apoptosis-regulator )兔抗人TIGAR蛋白抗體                                   

QY-18702       Anti-TIMP-1(Tissue Inhibitor of Metalloproteinase-1)金屬蛋白酶組織抑制因子-1抗體                                   

QY-18712       Anti-TIMP-2(Tissue Inhibitor of Metalloproteinase-2)金屬蛋白酶組織抑制因子-2抗體                                   

QY-18722       Anti-TIMP-3(Tissue Inhibitor of Metalloproteinase-3)金屬蛋白酶組織抑制因子-3抗體                                   

QY-18732       Anti-TIMP-4(Tissue Inhibitor of Metalloproteinase-4)金屬蛋白酶組織抑制因子-4抗體                                   

QY-18742       Anti-TLR2 (Toll-like receptor 2)Toll樣受體2抗體                                       

QY-18752       Anti-TLR4 (Toll-like receptor 4)Toll樣受體4抗體                                       

QY-18762       Anti-TLR5/CD285 (Toll-like receptor 5)Toll樣受體5抗體                                   

QY-18772       Anti-TM（Thrombomodulin)血栓調(diào)節(jié)蛋白抗體                                   

QY-18782       Anti-TmR2（GDNFRβ；TRN-R；GFR-alpha-2；NTNR-2）膠質(zhì)細(xì)胞系源性神經(jīng)營養(yǎng)因子受體β抗體                                    【大鼠粒細(xì)胞集落刺激因子(G-CSF)ELISA試劑盒現(xiàn)貨】

QY-18792       anti-Tn-C(tenascin-C)腱糖蛋白-C抗體                                         

QY-18802       Anti-TNF-α (Mouse Anti-Human Tumor Necrosis Factor-α Monoclonal Antibody)鼠抗人腫瘤壞死因子-α單克隆抗體                                      

QY-18812       Anti-TNF-α(Tumor Necrosis Factor-α)腫瘤壞死因子-α抗體                                        

QY-18822       Anti-TNF-β(Tumor Necrosis Factor-β)腫瘤壞死因子-β抗體                                        

QY-18832       Anti-TPⅠ （topoisomeraseⅠ）拓普西異構(gòu)酶Ⅰ抗體                                       

QY-18842       Anti-DNA TPⅡα (DNA topoisomeraseⅡα)DNA拓普西異構(gòu)酶Ⅱ抗體                                         

QY-18852       Anti-TPH (Trptophan Hydroxylase)色氨酸羥化酶抗體                                        

QY-18862       Anti-TPO (Thyroid Peroxidase)抗甲狀腺過氧化物酶抗體                                   

QY-18872       Anti-TRA16 (Testicular orphan nuclear receptor-4 (TR4)-associated protein)睪丸激素受體相關(guān)蛋白16抗體                                        

QY-18882       Anti-TRA16 (Testicular orphan nuclear receptor-4 (TR4)-associated protein)睪丸激素受體相關(guān)蛋白/孤兒素受體相關(guān)蛋白抗體                                      

QY-18892       Anti-TRADD （TNF receptor 1 associated via death domain）有死亡區(qū)的腫瘤壞死因子受體1相關(guān)蛋白抗體                                   

QY-18902       Anti-TRAF1 （TNF receptor-associated factor 1）腫瘤壞死因子受體相關(guān)因子1抗體                                         

QY-18912       Anti-TRAF2 （TNF receptor-associated factor 2）腫瘤壞死因子受體相關(guān)因子2抗體                                         

QY-18922       Anti-TRAF3/CD40bp （CD40 binding protein）CD40結(jié)合蛋白/CD40受體相關(guān)因子1抗體                                         

QY-18932       Anti-TRAF6 （TNF receptor-associated factor 6）腫瘤壞死因子受體相關(guān)因子6抗體                                         

QY-18942       Anti-TRAIL/Apo2L (tumor necrosis factor-related apoptosis-inducingligand)腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體/凋亡素2配體抗體                                          【大鼠粒細(xì)胞集落刺激因子(G-CSF)ELISA試劑盒現(xiàn)貨】

QY-18952       Anti-Transthyretin (TTR/prealbumin)轉(zhuǎn)甲狀腺素蛋白抗體                                         

QY-18962       Anti-TRBF1 (Telomeric repeat binding factor 1)端粒體復(fù)制結(jié)合因子1抗體                                     

QY-18972       Anti-TRF1（telomere repeat binding factor1）端粒結(jié)合蛋白1抗體                                   

QY-18982       Anti-TrK A (Tyrosine kinase,TrkA)酪氨酸激酶A抗體(神經(jīng)生長因子受體的一種)