【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計算
齊一生物供應(yīng)ELISA試劑盒檢測的目的是為實驗提供準(zhǔn)確可靠的定量分析實驗依據(jù)。為了保證實驗數(shù)據(jù)的可靠性，在實驗過程中必須堅持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個完整的計劃。

【小鼠細胞周期素D3(Cyclin-D3)ELISA試劑盒價格】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【小鼠細胞周期素D3(Cyclin-D3)ELISA試劑盒價格】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍色）

3.動物組織勻漿（細胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測細胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【小鼠細胞周期素D3(Cyclin-D3)ELISA試劑盒價格】樣本離心轉(zhuǎn)速及時間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【小鼠細胞周期素D3(Cyclin-D3)ELISA試劑盒價格】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9238685  Mains switch ETA, 10 A              CNY                            【小鼠細胞周期素D3(Cyclin-D3)ELISA試劑盒價格】

9231656  Holder, CCD Camera BL-185        CNY                           

9231538  Needle, W-Drive, DISU, BR8        CNY                           

9014876  Microplate Station Adapter            CNY                           

9014556  Shaker Adapter 8000  24-tubes  13ml              CNY                           

9014499  Disposable Tip Station, BR8, RD v1.0         CNY                           

9022574  Strut assembly, Hood, SP, right, Qsym        CNY                           

9238348  Upgrade, Precision Pippeting Precision pipetting upgrade includes onsite installation ａnd calibration visit including all travel ａnd labor to install the precision pipetting system, as well as the following components: 4x100ul syringes, 4x 3 Port Valves, 24x96 10ul Disposable Tips ａnd a precision Pipetting Calibration Kit.    CNY                           

9022379  Notebook, RGQ            CNY                           

9021984  Transport lock, QXA            CNY                           

9021892  Bracket, optical sensor, angled, QIAC          CNY                           

9021613  Kit, upgrade QSSP Cabinet, Qsym        CNY                           

9238605  Centrifuge Motor, complete          CNY                           

9238552  RS232 box, online fill ready, RapidPlate              CNY                           

9238253  Syringe Seal           CNY                           

9238222  De-Bubbler Top             CNY                           

9240674  Clamp Kit, Sensor, Reagent Jar, AP96/196           CNY                            【小鼠細胞周期素D3(Cyclin-D3)ELISA試劑盒價格】

9240665  Nut, Flangeless,1/4-28, White, AP        CNY                           

9240656  Rebuild Kit, Gast Pump, AP96             CNY                           

9240646  External BCR, 2D, HHP, AP          CNY                           

9240619  Fuse, TE5, 2.0A, PCB, AP96/196         CNY                           

9240610  Hall Effects Sensor, Latch Lock, AP98        CNY                           

9240609  Fiber Optic Sensor, Sub-unit, AP98             CNY                           

9240587  Vacuum Switch, Pump, AP96              CNY                           

9240579  Vacuum Switch Assy, Shaker, AP96            CNY                           

9240577  Cable Assy, Capper, X52 CW, AP96/196             CNY                           

9240525  Cable, Motor Axis #2, Elevator, AP98          CNY                           

9240518  Communication Module, RS232-485, AP196              CNY                           

9240502  PM Kit, AP96         CNY                           

9240348  Autopure, Premium Agreement     The Premium Cover Agreement Autopure provides full service coverage for the Autopure LS System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).  CNY                           

9240241  Housing, disposable tip adapter, TR             CNY                           

9239957  EZ1, PM Service    Service covers all travel ａnd labor costs plus all necessary parts to perform a Preventative Maintenance. The instrument will also be thoroughly checked ａnd inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed.   CNY                           

QY-02050       GHRF (GHRH)生長激素釋放激素（因子）（抗原）         0.5mg/6501mg/1100                         

QY-08000       ANGPTL1/ANG-1 (Angiopoietin-like Protein 1)血管位蛋白樣1/血管生成素樣蛋白-1抗原           0.5mg/6501mg/1100                         

QY-03840       GLUT4(Glucose transporter type 4）葡萄糖轉(zhuǎn)運蛋白4（抗原）            0.5mg/6501mg/1100                          【小鼠細胞周期素D3(Cyclin-D3)ELISA試劑盒價格】

QY-00980       GIP （Gastric Inhibitory polypeptide）胃泌素抑制肽（抗原）               0.5mg/6501mg/1100                         

QY-02080       GLP-2（Glucagon-like peptide-2）胰高血糖素樣肽-2（多肽抗原）               0.5mg/6501mg/1200                         

QY-01940       GRP94 （gp96）94kDa 糖調(diào)節(jié)蛋白（抗原）          0.5mg/6501mg/1100                         

QY-03480       G Protein(Guanine nucleotide-binding regulatory protein)G蛋白（鳥苷酸結(jié)合蛋白）（抗原）              0.5mg/6501mg/1100                         

QY-02520       GR (Glucocorticoid receptor)糖皮質(zhì)激素受體 多肽          0.5mg/6501mg/1100                         

QY-00230       GSK-3β(NT) (Glycogen Synthase Kinase-3β)糖原合酶激酶-3β（抗原）           0.5mg/6501mg/1100                         

QY-03410       CKR-L2（ G protein-coupled receptor-2 ）G蛋白偶聯(lián)受體-2(抗原)             0.5mg/6501mg/1100                         

QY-00280       GSK-3β, (Glycogen Synthase Kinase-3)葡萄糖合成激酶-3（抗原）             0.5mg/6501mg/1100                         

QY-01360       GTP-CH-1三磷酸鳥苷環(huán)水解酶（抗原）         0.5mg/6501mg/1100                         

QY-03440       H5N1-M2（Avian influenza Matrix Protein-2）H5N1流感病毒M2型（抗原）              0.5mg/6501mg/1100                         

QY-02210       HCV-Core丙型肝炎病毒-C區(qū)（多肽）             1mg/7002mg/1300                           

QY-02180       HCV-NS1丙型肝炎病毒-NS1（抗原）              0.5mg/6501mg/1100                         

QY-02190       HCV-NS3丙型肝炎病毒-NS3（抗原）              0.5mg/10001mg/1800                       

QY-02130       HCV-NS4a丙型肝炎病毒-NS4a（抗原）           0.5mg/10001mg/1800                       

QY-02120       HEV戊型肝炎病毒（抗原）               0.5mg/6501mg/1100                         

QY-03380       HGV（Hepatitis G vivus）庚型肝炎病毒（抗原）            0.5mg/6501mg/1100                          【小鼠細胞周期素D3(Cyclin-D3)ELISA試劑盒價格】

QY-02870       HisX6聚組氨酸標(biāo)簽蛋白             0.5mg/8001mg/1400                         

QY-02390       HIV-1(gp41)艾滋病病毒（抗原）               0.5mg/6501mg/1100                         

QY-02410       HIV gp120艾滋病病毒（抗原）         0.5mg/6501mg/1100                         

QY-01910       HSP-60（ Heat shock protein-60）熱休克蛋白-60（抗原）            0.5mg/6501mg/1100                         

QY-01260       HSP-70 (Heat shock protein-70)熱休克蛋白-70（多肽抗原）          0.5mg/6501mg/1100                         

QY-02140       ICAD（Inhibitor of Caspase-Activated DNase ）Caspase 激活的脫氧核糖核酸酶抑制劑（抗原）            0.5mg/6501mg/1100                         

QY-00180       IDE, (Insulin degradation enzyme)胰島素降解酶（抗原）               0.5mg/6501mg/1100                         

QY-02270       IGF-1 R 人胰島素樣生長因子-I受體         0.5mg/6501mg/1100                         

QY-04060       IGFBP-5 ( Insulin-like Growth Factor Binding Protein 5、IBP5)胰島素樣生長因子結(jié)合蛋白-5(抗原)          0.5mg/6501mg/1100                         

QY-00560       Isulin胰島素（抗原）          0.5mg/6501mg/1100                         

QY-08490       apoA1 (Apolipoprotein/APOA1 protein )human載脂蛋白A1抗原             0.5mg/6501mg/1100