【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準備試劑，樣品和標準品

2.加入準備好的樣品和標準品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計算
齊一生物供應(yīng)ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據(jù)。為了保證實驗數(shù)據(jù)的可靠性，在實驗過程中必須堅持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標本前都必須有一個完整的計劃。

【小鼠牛血清白蛋白（BSA)ELISA試劑盒】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【小鼠牛血清白蛋白（BSA)ELISA試劑盒】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍色）

3.動物組織勻漿（細胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標

              1g組織加入1ml組織裂解液加入8ml PBS 測細胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【小鼠牛血清白蛋白（BSA)ELISA試劑盒】樣本離心轉(zhuǎn)速及時間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【小鼠牛血清白蛋白（BSA)ELISA試劑盒】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

一個給定的分析物的量可能會有所不同，不僅從物種的物種，但也組織和細胞來源。這個信息的*佳來源是目前的文獻，很容易通過互聯(lián)網(wǎng)在多個科學數(shù)據(jù)庫訪問。

18。  我光密度稍高（或低）比，是與我的試劑盒說明書。為什么？

試劑盒的光密度的物理條件，如時間和溫度的影響。我們建議您縮短或延長*終孵化與基板解決方案，以補償。

【小鼠基質(zhì)金屬蛋白酶2/明膠酶A(MMP-2/GelatinaseA)ELISA試劑盒】19。  高背景的原因是什么？

1）     洗滌不當：檢查洗滌液儲量和確保所有建議的洗滌步驟進行。

2）     污染基材：確保沒有污染的金屬離子或氧化劑的基質(zhì)，使用前。在酶聯(lián)免疫吸附底物的開發(fā)時間內(nèi)，保持額外的底物溶液。

3）暴露于光     基材：曝光可能會導致在一個藍色的基板。在黑暗中保存（瓶）的解決方案，直到準備好進入板。

4）     錯的孵育時間/溫度：一般按照測試協(xié)議對于培養(yǎng)時間和溫度。然而，如果所有的威爾斯是強烈的和同樣顏色的標準稀釋系列中觀察到的強度梯度，那么它可能是必要的觀察到的顏色的底物反應(yīng)，以阻止反應(yīng)更快。

9241768QIAxtractor, Basic AgreementThe Basic Cover Agreement, QIAxtractor provides full service coverage for the QIAxtractor ａnd the CAS 1280. Costs for service visits are fully covered including travel, labor, ａnd parts. One on-site pipetting head service ａnd inspection is included. With a Basic Cover Agreement, there is a response time of 5 working days.CNY

9241656Transfer Rack Plate, APCNY

9241630Shaker Adjustment Kit, APCNY

9241367Damper, door, EZ1aCNY

9240759Update, Sliding Door Latch, AP98CNY

9243548Pyro Q96 ID, PM Service"Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"CNY

9242766Cogwheel, x-drive, portal, QsymCNY

9242710Validation Support, 2d, QIAgilityThe QIAGEN QIAgility Guided Validation Support service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the QIAgility system ａnd software, review the User Manual, ａnd practical hands-on operation. The Guided Validation service includes experimental design consultation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which processes (quant setup, normalization, PCR setup, CE setup) ａnd kit manufacturer (Applied Biosystems, Promega, QIAGEN) need to be included in the validation.CNY

9242253Cable, x, portal AS, QsymCNY

9242234RGQ, IQ/OQ Service"Instrument qualification support service for the Rotorgene Q ａnd Rotorgene 6000. The product contains the IQ/OQ documentation ａnd the performance of the qualification protocols (travel ａnd labor costs included). 

During the service, the compliance with the manufacturer＇s installation requirements is testified ａnd the performance verification & dynamic calibration of the Rotorgene Q is performed."CNY

9242172Repair fee, Depot Repair, TissueLyser IICNY

5000-1403Bumpers Side, VTX2CNY

5000-1401Guide Rack Back Asmbly, VTX2CNY

5000-1311Capacitor 8UF, SKR1CNY

5000-1273Door Damper Assembly, DML2CNY

5000-1258Y Optical Sensor CMPLT, DML2CNY

5050-1074 V.1CD Rom Manual - LumiCheck PlateCNY

5050-1069 V.1CD Rom Manual - Rotary Shaker I, 240VCNY

6000-5001Costar Plate LidsCNY

6000-3120Plate Washer I - WasteReserv.CapAssemblyCNY

5000-1420Driveplate Asmly 099A, VTX2CNY

5127-1220hc2 Sample Conversion KitCNY

9243701careHPV, TrainingCNY

EA11423EASYartus BKV RG_ICNY

800190Sequencing in a Box ServiceCNY

800110Single Read, w/ prep"Bacterial clone cultivation ａnd template purification or PCR product purification, including DNA quality control

Repetition of sequencing, if necessary

Data collected to maximal read length"CNY

132538NeXtal Stock Dioxane (50)100% v/v Dioxane - 50mL (5 x 10 ml tube)CNY

338162RT2 PCR Array Loading ReservoirCNY

338114Mouse XpressRef Universal Total RNACNY

203743QIAGEN Fast Cycling PCR Kit (200)For 200 x 20 µl reactions: 2 x 1 ml Fast Cycling Master Mix, 2x (containing dNTPs, patent pending buffer composition), RNase-free water, 10 x CoralLoad Fast Cycling Dye, 5 x Q-SolutionCNY