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技術(shù)文章

MCM7 antibody Catalog Number：OM262672

點(diǎn)擊次數(shù)：49 發(fā)布時(shí)間：2024/9/27 14:59:40
 

Product Profile

Product Name MCM7 antibody
Antibody Type Primary Antibodies
Product description 
Mouse monoclonal [47DC141] to MCM7hCDC47 is a human member of the MCM family, which allows DNA to replicate once per cell cycle. In quiescent cells, human MCM7 (hMCM7) mRNA is almost undetectable. Stimulation of cells to enter the cell cycle results in induction of hMCM7 expression. The hCDC47 protein expression ａnd localization is found in nuclei of the proliferative components of normal lymph nodes, bone marrow, epidermis ａnd mucosa. Malignant tumors from several organs contained more nuclearhCDC47 positive cells than their normal counterparts. These results indicate that hCDC47 may play a role in normal ａnd neoplastic cell growth in vivo.
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Immunogen 
Full length recombinant human MCM7

Key Feature

Clonality Monoclonal
Isotype IgG1
Clone Number 47DC141
Host Species Mouse
Tested Applications

ICC/IFIHC-PIPWB
 
ICC/IF:1:20~1:100
IHC-p:1:10~1:50
WB:1:100~1:200
IP:1:20~1:100
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Species Reactivity

HumanMouseDogRatXenopus
Concentration 0.2 mg/ml
Purification Affinity purified

Target Information

Gene Synonyms
CDC47
MCM2
P1.1-MCM3
P1CDC47
P85MCM
PNAS146
P1.1 MCM3
P1.1MCM3
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Target MCM7
Cellular Localization Nuclear .

Application

  • Application

    Formalin fixed paraffin embedded human tonsil stained with MCM7, using ABC ａnd DAB chromagen.

  • Application

    Formalin-fixed, paraffin-embedded human tonsil stained with using peroxidase-conjugate ａnd DAB chromogen. Note nuclear staining of proliferating cells.

Positive Control Breast carcinoma
Application Notes
ICC/IF:1:20~1:100
IHC-p:1:10~1:50
WB:1:100~1:200
IP:1:20~1:100
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Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution, aliquot ａnd store at 4°C. Do not freeze.
Storage Buffer PBS, pH7.4 containg 0.2% BSA ａnd 0.09% sodium azide

原創(chuàng)作者：上海篤瑪生物科技有限公司

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