Sterilization Method

Filtration

Extraction Method

Enzyme - atelocollagen

Form

Solution

Package Size

20 mL, 100 mL

Storage Temperature

2-10°C

Shelf Life

Minimum of 6 months from date of receipt

Collagen Concentration - Biuret

2.9-3.2 mg/mL

Collagen Purity - Silver Staining

99%

pH

1.9-2.1

Kinetic Gel Test (Minutes)

40

Gel Formation Tube Test (Minutes)

40

Fibrillogenesis (Absorbance Units)

0.5

Electrophoretic Pattern - Coomassie Blue

Characteristic

Sterility - USP modified

No growth

Endotoxin - LAL

5.0 EU/mL

Osmolality (mOsmo H2O/kg)

35

Cell Attachment Assay

Pass

Source

Human Neo-Natal Fibroblasts

Hydrogel Young s Modulus E (Pa)

Characteristic

Product References

References for VitroCol®:

Shieh, Hester F., et al. Comparisons of human amniotic mesenchymal stem cell viability in FDA-approved collagen-based scaffolds: Implications for engineered diaphragmatic replacement. Journal of pediatric surgery 52.6 (2017): 1010-1013.

van der Velden, Jos LJ, et al. Transforming Growth Factor-? Induces A Mesenchymal Profile In Human Nasal Epithelial Cells. D76. ALVEOLAR EPITHELIUM: NOVEL TOOLS AND PHENOTYPES. American Thoracic Society, 2012. A6322-A6322.

Tashima, Takumi, et al. Osteomodulin regulates diameter ａnd alters shape of collagen fibrils. Biochemical ａnd biophysical research communications 463.3 (2015): 292-296.

Spiller, Kara L., Suzanne A. Maher, ａnd Anthony M. Lowman. Hydrogels for the repair of articular cartilage defects. Tissue engineering part B: reviews 17.4 (2011): 281-299.

Brilha, Sara, et al. Monocyte adhesion, migration, ａnd extracellular matrix breakdown are regulated by integrin αVβ3 in Mycobacterium tuberculosis infection. The Journal of Immunology 199.3 (2017): 982-991.

Jonsdottir, Hulda R., ａnd Ronald Dijkman. Characterization of human coronaviruses on well-differentiated human airway epithelial cell cultures. Coronaviruses. Humana Press, New York, NY, 2015. 73-87.

Sabbione, Florencia, et al. Neutrophil extracellular traps stimulate proinflammatory responses in human airway epithelial cells. Journal of innate immunity 9.4 (2017): 387-402.

Dos Santos Brilha, S., et al. Monocyte adhesion, migration ａnd extracellular matrix breakdown is regulated by integrin αVβ3 in Mycobacterium tuberculosis infection.

Brilha, Sara, et al. Monocyte Adhesion, Migration, ａnd Extracellular Matrix Breakdown Is Regulated by Integrin aVb3 in Mycobacterium tuberculosis Infection. (2017).

Colace, T., et al. Analysis of morphology of platelet aggregates formed on collagen under laminar blood flow. Annals of biomedical engineering 39.2 (2011): 922-929.

Muthard, Ryan W., ａnd Scott L. Diamond. Blood clots are rapidly assembled hemodynamic sensors: flow arrest triggers intraluminal thrombus contraction. Arteriosclerosis, thrombosis, ａnd vascular biology 32.12 (2012): 2938-2945.

Maloney, S. F., Lawrence F. Brass, ａnd S. L. Diamond. P2Y12 or P2Y1inhibitors reduce platelet deposition in a microfluidic model of thrombosis while apyrase lacks efficacy under flow conditions. Integrative Biology 2.4 (2010): 183-192.

Bauer, Rebecca N., et al. Interaction with epithelial cells modifies airway macrophage response to ozone. American journal of respiratory cell ａnd molecular biology 52.3 (2015): 285-294.

Kawamura, Shunsuke, et al. Identification of common monocyte progenitors, pre-monocytes, ａnd granulocyte monocyte progenitors in human umbilical cord blood. Experimental Hematology 43.9 (2015): S72.